Gong Nian, Yang Lin, Chen Xiangsheng
Guizhou University, Institute of Entomology, Guiyang, Guizhou, China.
Guizhou University, The Provincial Special Key Laboratory for Development and Utilization of Insect Resources, Guiyang, Guizhou, China.
PeerJ. 2021 Nov 16;9:e12465. doi: 10.7717/peerj.12465. eCollection 2021.
Here, the complete mitochondrial genomes (mitogenomes) of 12 Caliscelidae species, , , , , , , , , , , , and sp., were determined and comparatively analyzed. The genomes varied from 15,424 to 16,746 bp in size, comprising 37 mitochondrial genes and an A+T-rich region. The typical gene content and arrangement were similar to those of most Fulgoroidea species. The nucleotide compositions of the mitogenomes were biased toward A/T. All protein-coding genes (PCGs) started with a canonical ATN or GTG codon and ended with TAN or an incomplete stop codon, single T. Among 13 PCGs in 16 reported Caliscelidae mitogenomes, and showed the lowest and highest nucleotide diversity, respectively. All PCGs evolved under purifying selection, with considered a comparatively fast-evolving gene. Phylogenetic relationships were reconstructed based on 13 PCGs in 16 Caliscelidae species and five outgroups using maximum likelihood and Bayesian inference analyses. All species of Caliscelidae formed a steadily monophyletic group with high support. Peltonotellini was present at the basal position of the phylogenetic tree. Augilini was the sister group to Caliscelini and Peltonotellini.
在此，测定并比较分析了12种扁蜡蝉科物种（分别为 、 、 、 、 、 、 、 、 、 、 、 以及 属）的完整线粒体基因组（线粒体基因组）。这些基因组大小在15424至16746 bp之间，包含37个线粒体基因和一个富含A+T的区域。典型的基因组成和排列与大多数蜡蝉总科物种相似。线粒体基因组的核苷酸组成偏向于A/T。所有蛋白质编码基因（PCGs）均以典型的ATN或GTG密码子起始，并以TAN或不完整的终止密码子（单个T）结束。在已报道的16个扁蜡蝉科线粒体基因组的13个PCGs中， 和 分别显示出最低和最高的核苷酸多样性。所有PCGs都在纯化选择下进化， 被认为是一个进化相对较快的基因。基于16种扁蜡蝉科物种的13个PCGs和5个外类群，使用最大似然法和贝叶斯推断分析重建了系统发育关系。所有扁蜡蝉科物种形成了一个得到高度支持的稳定单系类群。佩尔顿诺特叶蝉族位于系统发育树的基部位置。奥吉叶蝉族是卡利叶蝉族和佩尔顿诺特叶蝉族的姐妹群。
