Kim Iksoo, Jeong Su Yeon, Kim Min Jee
Department of Applied Biology, College of Agriculture and Life Sciences, Chonnam National University, Gwangju, Republic of Korea.
Mitochondrial DNA B Resour. 2019 Jul 11;4(2):2201-2203. doi: 10.1080/23802359.2019.1624216.
The dragonfly Selys, 1883 (Odonata: Libellulidae) has been listed as a critically endangered species by the International Union for Conservation of Nature (IUCN) and is also an endangered insect in South Korea. We sequenced the whole genome (15,233 bp) of species, which included a set of typical genes and one major non-coding AT-rich region with an arrangement identical to that observed in most insect genomes. The A + T-rich region harbored one identical repeat composed of 65 bp and two tRNA-like structures ( and -like sequences) with proper anticodon and clover-leaf structures. Phylogenetic reconstruction using the concatenated sequences of 13 protein-coding genes (PCGs) and two rRNAs of the representative odonate mitogenomes utilizing both Bayesian inference and maximum-likelihood methods revealed a strong support for the monophyletic Zygoptera and a moderate to high support for the monophyletic Anisoptera suborders. Unlike that in conventional phylogenetic analysis, a relatively strong sister relationship was revealed between the suborders of Anisozygoptera and Zygoptera.
1883年的蜻蜓（蜻蛉目：蜻科）被国际自然保护联盟（IUCN）列为极度濒危物种，在韩国也是濒危昆虫。我们对该物种的全基因组（15233 bp）进行了测序，其中包括一组典型基因和一个主要的富含AT的非编码区域，其排列与大多数昆虫基因组中观察到的相同。富含A+T的区域包含一个由65 bp组成的相同重复序列和两个具有适当反密码子和三叶草叶结构的类似tRNA的结构（和-like序列）。利用贝叶斯推断和最大似然法，对代表性蜻蛉目线粒体基因组的13个蛋白质编码基因（PCGs）和两个rRNA的串联序列进行系统发育重建，结果显示对单系的均翅亚目有很强的支持，对单系的差翅亚目有中等至高的支持。与传统系统发育分析不同的是，在间翅亚目和均翅亚目之间发现了相对较强的姐妹关系。
