Song Jinfang, Zhuang Yongru, Pan Xiaojun, Chen Ya, Xie Fen
Department of Pharmacy, Affiliated Hospital of Jiangnan University, Wuxi, 214000, China.
Jiangsu Key Laboratory of New Drug Research and Clinical Pharmacy, Xuzhou Medical University, Xuzhou, 221000, China.
Heliyon. 2024 Jul 26;10(15):e35289. doi: 10.1016/j.heliyon.2024.e35289. eCollection 2024 Aug 15.
Genetic susceptibility is an important pathogenic mechanism in diabetic kidney disease (DKD). Our previous studies have identified that PPARδ and GLP-1R are located in a pathway that is closely related to DKD. We aimed to explore the impacts of variants in - on the susceptibility to DKD in Chinese Han patients with type 2 diabetes mellitus (T2DM). A total of 600 T2DM patients (300 with DKD and 300 without DKD) and 200 healthy control subjects were enrolled to identify (rs2016520, rs2267668 and rs3777744) and (rs3765467, rs1042044 and rs9296291) genotype. The SNaPshot method was used to identify variants in -. We performed correlation analysis between variants in - and the susceptibility to DKD. We observed that rs3765467 (G > A) was associated with DKD (OR = 3.145, 95 % CI = 2.128-6.021, P = 0.035). None of the other SNPs were associated with DKD. Regarding DKD related traits, rs3765467 was associated with UACR levels and TC, significant differences were observed among patients with different genotypes of rs2016520 in terms of BMI and TG, and patients with the rs3777744 risk G allele had noticeably higher PPG and HbA1c levels (P < 0.05). Moreover, the results showed the interactions between rs3777744 and rs3765467 in the occurrence of DKD (OR = 4.572, P = 0.029). The results of this study indicate the potential relationship between variants in - and the susceptibility to DKD in Chinese Han patients with T2DM.
遗传易感性是糖尿病肾病（DKD）的一种重要致病机制。我们之前的研究已经确定，过氧化物酶体增殖物激活受体δ（PPARδ）和胰高血糖素样肽-1受体（GLP-1R）位于一条与DKD密切相关的信号通路中。我们旨在探讨[相关基因区域]的变异对中国汉族2型糖尿病（T2DM）患者DKD易感性的影响。共纳入600例T2DM患者（300例患有DKD，300例未患DKD）和200例健康对照者，以鉴定[相关基因]（rs2016520、rs2267668和rs3777744）和[另一相关基因]（rs3765467、rs1042044和rs9296291）的基因型。采用SNaPshot方法鉴定[相关基因区域]的变异。我们对[相关基因区域]的变异与DKD易感性进行了相关性分析。我们观察到rs3765467（G>A）与DKD相关（比值比[OR]=3.145，95%置信区间[CI]=2.128 - 6.021，P=0.035）。其他单核苷酸多态性（SNP）均与DKD无关。关于DKD相关特征，rs3765467与尿白蛋白肌酐比值（UACR）水平和总胆固醇（TC）相关，rs2016520不同基因型患者在体重指数（BMI）和甘油三酯（TG）方面存在显著差异，rs3777744风险G等位基因的患者餐后血糖（PPG）和糖化血红蛋白（HbA1c）水平明显更高（P<0.05）。此外，结果显示rs3777744与rs3765467在DKD发生中存在相互作用（OR=4.572，P=0.029）。本研究结果表明[相关基因区域]的变异与中国汉族T2DM患者DKD易感性之间存在潜在关系。
