Faculty of Medicine and Biomedical Sciences, Autonomous University of Chihuahua, Circuito Universitario, Campus II, Chihuahua 31109, Mexico.
Faculty of Chemical Sciences, Autonomous University of Chihuahua, Circuito Universitario, Campus II, Chihuahua 31109, Mexico.
Genes (Basel). 2020 Jul 10;11(7):776. doi: 10.3390/genes11070776.
Peroxisome proliferator-activated receptors (PPARs) play roles in glucose and lipid metabolism regulation. Pro12Ala PPAR-γ2 and +294T/C PPAR-δ have been associated with dyslipidemia, hyperglycemia and high body mass index (BMI). We compared metabolic traits and determined associations with Pro12Ala PPAR-γ2 or +294T/C PPAR-δ polymorphism among teenagers from different ethnicity. Four hundred and twelve samples with previous biochemical and biometric measurements were used. Genomic DNA from peripheral blood was extracted and analyzed by end-point PCR for Pro12Ala PPAR-γ2. The +294T/C PPAR-δ PCR product was also digested with Bsl I. Two genotype groups were formed: major allele homozygous and minor allele carriers. Pro12Ala PPAR-γ2 G minor allele frequencies were: 10% in Mestizo-1, 19% in Mestizo-2, 23% in Tarahumara, 12% in Mennonite, and 17% in the total studied population. The +294T/C PPAR-δ C minor allele frequencies were: 18% in Mestizo-1, 20% in Mestizo-2, 6% in Tarahumara, 13% in Mennonite, and 12% in the total studied population. Teenagers with PPAR-γ2 G allele showed a greater risk for either high waist/height ratio or low high-density lipoprotein; and, also had lower total cholesterol. Whereas, PPAR-γ2 G allele showed lower overweight/obesity phenotype (BMI Z-score) frequency, PPAR-δ C allele was a risk factor for it. Metabolic traits were associated with both PPAR polymorphisms.
过氧化物酶体增殖物激活受体 (PPARs) 在葡萄糖和脂质代谢调节中发挥作用。Pro12Ala PPAR-γ2 和 +294T/C PPAR-δ 与血脂异常、高血糖和高体重指数 (BMI) 有关。我们比较了不同种族青少年的代谢特征，并确定了与 Pro12Ala PPAR-γ2 或 +294T/C PPAR-δ 多态性的关联。使用了 412 个具有先前生化和生物计量测量值的样本。从外周血中提取基因组 DNA，并通过终点 PCR 分析 Pro12Ala PPAR-γ2。+294T/C PPAR-δ PCR 产物也用 Bsl I 消化。形成了两种基因型组：主要等位基因纯合子和次要等位基因携带者。Pro12Ala PPAR-γ2 G 次要等位基因频率为：10%在梅斯蒂索-1 中，19%在梅斯蒂索-2 中，23%在塔拉乌马拉中，12%在门诺派教徒中，17%在总研究人群中。+294T/C PPAR-δ C 次要等位基因频率为：18%在梅斯蒂索-1 中，20%在梅斯蒂索-2 中，6%在塔拉乌马拉中，13%在门诺派教徒中，12%在总研究人群中。携带 PPAR-γ2 G 等位基因的青少年患高腰围/身高比或低高密度脂蛋白的风险更大；并且，总胆固醇也较低。然而，PPAR-γ2 G 等位基因显示出较低的超重/肥胖表型（BMI Z 分数）频率，而 PPAR-δ C 等位基因是其危险因素。代谢特征与两种 PPAR 多态性相关。
