Kaczmarek-Szczepańska Beata, Glajc Patrycja, Chmielniak Dorota, Gwizdalska Klaudia, Swiontek Brzezinska Maria, Dembińska Katarzyna, Shinde Ambika H, Gierszewska Magdalena, Łukowicz Krzysztof, Basta-Kaim Agnieszka, D'Amora Ugo, Zasada Lidia
Department of Biomaterials and Cosmetics Chemistry, Faculty of Chemistry, Nicolaus Copernicus University in Torun, Gagarina 7, 87-100 Torun, Poland.
Department of Environmental Microbiology and Biotechnology, Faculty of Biological and Veterinary Sciences, Nicolaus Copernicus University in Torun, Lwowska 1, 87-100 Torun, Poland.
ACS Appl Mater Interfaces. 2025 Mar 12;17(10):15196-15207. doi: 10.1021/acsami.5c00715. Epub 2025 Feb 25.
Chitosan (CTS) has emerged as a promising biopolymer for wound healing due to its biocompatibility, biodegradability, and intrinsic bioactive properties. This study explores the development and characterization of CTS-based films enhanced with natural bioactive agents, aloe vera (A), gluconolactone (GL), and sorbitol (S), to improve their mechanical, antimicrobial, and regenerative performance for potential use in advanced wound care. A series of CTS-based films were fabricated with varying concentrations of A, GL, and S, and their physicochemical, mechanical, and biological properties were comprehensively evaluated. Fourier transform infrared (FTIR) spectroscopy and atomic force microscopy (AFM) analysis revealed modifications in the film structure attributable to these additives, influencing the surface roughness, hydrophilicity, and thermal stability. Biocidal assays confirmed enhanced antimicrobial activity, particularly in films containing GL and A. Biodegradation studies demonstrated a significant enhancement in microbial decomposition of the films, while cytocompatibility tests confirmed minimal cytotoxic effects and improved cellular response. This research underscores the potential of combining CS with A, GL, and S to engineer multifunctional biomaterials tailored for effectively tackling different phases of the wound healing process, offering a sustainable and biocompatible alternative for clinical applications.
壳聚糖（CTS）因其生物相容性、生物可降解性和内在生物活性特性，已成为一种用于伤口愈合的有前景的生物聚合物。本研究探索了用天然生物活性剂芦荟（A）、葡萄糖酸内酯（GL）和山梨醇（S）增强的基于CTS的薄膜的开发与表征，以改善其机械性能、抗菌性能和再生性能，用于先进伤口护理的潜在用途。制备了一系列含有不同浓度A、GL和S的基于CTS的薄膜，并对其物理化学、机械和生物学性能进行了全面评估。傅里叶变换红外（FTIR）光谱和原子力显微镜（AFM）分析表明，这些添加剂导致了薄膜结构的改变，影响了表面粗糙度、亲水性和热稳定性。杀菌试验证实了抗菌活性的增强，特别是在含有GL和A的薄膜中。生物降解研究表明薄膜的微生物分解显著增强，而细胞相容性测试证实细胞毒性作用最小且细胞反应得到改善。本研究强调了将CS与A、GL和S结合以设计多功能生物材料的潜力，这些生物材料可有效应对伤口愈合过程的不同阶段，为临床应用提供了一种可持续且生物相容的替代方案。
