Development and Characterization of Biocompatible Chitosan-Aloe Vera Films Functionalized with Gluconolactone and Sorbitol for Advanced Wound Healing Applications.

Chitosan (CTS) has emerged as a promising biopolymer for wound healing due to its biocompatibility, biodegradability, and intrinsic bioactive properties. This study explores the development and characterization of CTS-based films enhanced with natural bioactive agents, aloe vera (A), gluconolactone (GL), and sorbitol (S), to improve their mechanical, antimicrobial, and regenerative performance for potential use in advanced wound care. A series of CTS-based films were fabricated with varying concentrations of A, GL, and S, and their physicochemical, mechanical, and biological properties were comprehensively evaluated. Fourier transform infrared (FTIR) spectroscopy and atomic force microscopy (AFM) analysis revealed modifications in the film structure attributable to these additives, influencing the surface roughness, hydrophilicity, and thermal stability. Biocidal assays confirmed enhanced antimicrobial activity, particularly in films containing GL and A. Biodegradation studies demonstrated a significant enhancement in microbial decomposition of the films, while cytocompatibility tests confirmed minimal cytotoxic effects and improved cellular response. This research underscores the potential of combining CS with A, GL, and S to engineer multifunctional biomaterials tailored for effectively tackling different phases of the wound healing process, offering a sustainable and biocompatible alternative for clinical applications.