Premchanth Jyothi Sreelekshmi, Sadanandan Sandhya, Rajamani Rejithamol
Department of Chemistry, Amrita Vishwa Vidyapeetham, Amritapuri, Kollam, India.
Department of Chemistry, School of Advanced Sciences, VIT-AP University, Amaravati, Andhra Pradesh, India.
Crit Rev Anal Chem. 2024 Aug 20:1-14. doi: 10.1080/10408347.2024.2390551.
In this era of emerging pathogenic diseases, prompt and accurate detection of pathogens is crucial. Disease diagnosis, environmental monitoring and food safety all rely heavily on the identification of pathogens. Peptide-based electrochemical sensors due to their rapid response times, specificity and sensitivity have emerged as promising tools in the identification of pathogens. This review emphasizes the importance of peptides in detection of pathogens and different peptide-based electrochemical biosensors for the detection of pathogens. Peptides offer several advantages including strong binding affinity to a diverse array of pathogens including bacteria, viruses and fungi, tunable specificity and simple synthesis. Peptide-based electrochemical sensors employ different electrochemical techniques such as differential pulse voltammetry (DPV), cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS), amperometry and linear sweep voltammetry (LSV). The efficacy of peptide-based biosensors in detecting low concentrations of pathogens is highlighted, demonstrating the promising applications of these biosensors in early diagnosis and real-time monitoring. In addition, the review also addresses the current challenges in the field such as peptide stability, sensor reproducibility and interference from complex biological matrices. This review suggests potential resolutions and avenues for progress such as the development of multiplexed detection systems that can concurrently identify multiple pathogens and developments in peptide design and sensor miniaturization. In summary, this review highlights the substantial advancements and potential possibilities of peptide-based electrochemical biosensors in the realm of pathogen detection, thereby facilitating the development of safer and more effective diagnostic tools.
在这个新发病原体不断出现的时代，快速准确地检测病原体至关重要。疾病诊断、环境监测和食品安全都严重依赖于病原体的鉴定。基于肽的电化学传感器因其快速的响应时间、特异性和灵敏度，已成为病原体鉴定中有前景的工具。本文综述强调了肽在病原体检测中的重要性以及用于病原体检测的不同基于肽的电化学生物传感器。肽具有多种优势，包括对包括细菌、病毒和真菌在内的多种病原体具有强结合亲和力、可调节的特异性和简单的合成方法。基于肽的电化学传感器采用不同的电化学技术，如差分脉冲伏安法（DPV）、循环伏安法（CV）、电化学阻抗谱（EIS）、安培法和线性扫描伏安法（LSV）。文中强调了基于肽的生物传感器在检测低浓度病原体方面的功效，展示了这些生物传感器在早期诊断和实时监测中的应用前景。此外，综述还讨论了该领域当前面临的挑战，如肽的稳定性、传感器的重现性以及复杂生物基质的干扰。本文综述提出了潜在的解决方案和发展途径，如开发能够同时鉴定多种病原体的多重检测系统，以及肽设计和传感器小型化方面的进展。总之，本文综述突出了基于肽的电化学生物传感器在病原体检测领域的重大进展和潜在可能性，从而推动更安全、更有效的诊断工具的开发。
