Tolba Mona Mohamed, Allam Amal Farahat, Khalil Safia Saleh, Elshouki Wagdy Makram, Shehab Amel Youssef
Department of Parasitology, Medical Research Institute, University of Alexandria, Alexandria, Egypt.
Department of Zoology, Faculty of Science, University of Alexandria, Alexandria, Egypt.
Trop Parasitol. 2022 Jul-Dec;12(2):87-93. doi: 10.4103/tp.tp_93_21. Epub 2022 Nov 24.
() diagnosis is an intestinal protozoan parasite globally found in rural and urban areas and is attracting a growing interest. Its prevalence in stool varies from 0.2% to more than 19% depending upon the population studied.
This study was based on the examination of 100 stool samples of randomly referred cases in a rural area in Motobus district, Kafr El-Sheikh governorate, Egypt. Our aim was to investigate the presence of in stool of the examined individuals using conventional polymerase chain reaction (PCR) compared to wet mount and trichrome stain with confirmation of infection by transmission electron microscopy.
was detected in 13/100 of the stool samples examined using wet mount smears, while trichrome stain detected 17/100. Conventional PCR diagnosed 41 cases of in the studied group. A very good agreement was found between wet mount and trichrome stain for diagnosing , while there was fair agreement between conventional PCR and both microscopy methods. Transmission electron microscope was performed on pooled positive samples that revealed the internal structures of trophozoite with its characteristic nucleus.
PCR technique was superior to microscopy for the detection of . Trichrome stain remains vital for microscopic diagnosis.
（某寄生虫名称）诊断是一种在全球农村和城市地区均有发现的肠道原生动物寄生虫，正引起越来越多的关注。其在粪便中的流行率因所研究的人群而异，从0.2%到超过19%不等。
本研究基于对埃及卡夫尔谢赫省莫托布斯区一个农村地区随机转诊病例的100份粪便样本进行检查。我们的目的是通过传统聚合酶链反应（PCR）与湿片检查和三色染色法相比，调查受检个体粪便中（该寄生虫）的存在情况，并通过透射电子显微镜确认感染情况。
使用湿片涂片法在100份受检粪便样本中的13份中检测到（该寄生虫），而三色染色法检测到17份。传统PCR在研究组中诊断出41例（该寄生虫感染）。在诊断（该寄生虫）方面，湿片检查和三色染色法之间有很好的一致性，而传统PCR与两种显微镜检查方法之间有较好的一致性。对合并的阳性样本进行了透射电子显微镜检查，揭示了（该寄生虫）滋养体及其特征性细胞核的内部结构。
PCR技术在检测（该寄生虫）方面优于显微镜检查。三色染色法对于显微镜诊断仍然至关重要。
