Peek Ron, Reedeker Freek R, van Gool Tom
Department of Medical Microbiology, Tropical Medicine and AIDS, University of Amsterdam, Amsterdam, The Netherlands.
J Clin Microbiol. 2004 Feb;42(2):631-5. doi: 10.1128/JCM.42.2.631-635.2004.
Dientamoeba fragilis is a globally occurring parasite that has been recognized as a causative agent of gastrointestinal symptoms. A single-round PCR was developed to detect D. fragilis DNA directly from human stool samples. The genetic diversity of D. fragilis from 93 patients and 6 asymptomatic carriers was examined by PCR followed by restriction fragment length polymorphism and sequencing of part of the small-subunit rRNA gene. The data show that D. fragilis sequences can be studied directly from fecal specimens despite the absence of a cyst stage and without the need for prior culturing. In addition, the results suggest strongly that D. fragilis shows remarkably little variation in its small-subunit rRNA gene.
脆弱双核阿米巴是一种在全球范围内存在的寄生虫，已被确认为胃肠道症状的病原体。开发了一种单轮聚合酶链反应（PCR），用于直接从人类粪便样本中检测脆弱双核阿米巴的DNA。通过PCR检测93例患者和6名无症状携带者的脆弱双核阿米巴的遗传多样性，随后进行限制性片段长度多态性分析，并对小亚基核糖体RNA基因的部分序列进行测序。数据表明，尽管没有包囊阶段且无需预先培养，但仍可直接从粪便标本中研究脆弱双核阿米巴序列。此外，结果强烈表明，脆弱双核阿米巴的小亚基核糖体RNA基因变异极小。
