Universidade Federal do Rio de Janeiro - Biomedical Engineering Program - Rio de Janeiro (RJ) - Brazil.
Universidade Federal do Delta do Parnaíba - Parnaíba (PI) - Brazil.
Acta Cir Bras. 2024 Aug 16;39:e394924. doi: 10.1590/acb394924. eCollection 2024.
This study aimed to evaluate a new therapeutic option for the spasticity using ultrasound neuromodulation in an animal model of spastic cerebral palsy.
Thirty-two adult male Wistar rats were randomly distributed in: negative control (NC); positive control (PC); untreated model (UTM); and treated model (TM). Rats in the control groups received sham surgery, and rats in the model groups received the spastic cerebral palsy model surgery. The rats' motor functions were evaluated by the Rotarod and CatWalk tests before and after surgery. PC and TM groups underwent ultrasonic neuromodulation by a physiotherapeutic ultrasound (intensity 0.1 W/cm2, at 1 MHz) continuous mode for 5 seconds, for seven days.
Twelve rats showed a spastic pattern (UTM = 6 and TM = 6), motor limitations (UTM = 6 and TM = 6), and ten had difficulty feeding (UTM = 5 and TM = 5). One UTM group rat could not recover its preoperative latency time, while the other rats in the model groups did. The speed at which the limbs swung reduced after surgery and increased in subsequent assessments, demonstrating greater instability and a deficit in locomotion balance.
Results were not yet sufficient to assert ultrasound neuromodulation as a possible therapy for spasticity in spastic cerebral palsy in the parameters used, and more studies are necessary.
本研究旨在评估一种新的治疗选择，即使用超声神经调节来治疗痉挛性脑瘫的动物模型中的痉挛。
32 只成年雄性 Wistar 大鼠被随机分为：阴性对照组（NC）；阳性对照组（PC）；未治疗模型组（UTM）；和治疗模型组（TM）。对照组大鼠接受假手术，模型组大鼠接受痉挛性脑瘫模型手术。在手术前后，通过转棒和 CatWalk 测试评估大鼠的运动功能。PC 和 TM 组接受了为期 7 天的连续模式的物理治疗超声（强度 0.1 W/cm2，1 MHz）超声神经调节，每次 5 秒。
12 只大鼠表现出痉挛模式（UTM = 6，TM = 6）、运动受限（UTM = 6，TM = 6）和 10 只大鼠出现喂养困难（UTM = 5，TM = 5）。一只 UTM 组大鼠无法恢复术前潜伏期时间，而模型组的其他大鼠则可以。手术后四肢摆动速度降低，随后评估时增加，表明运动平衡稳定性和缺陷更大。
在使用的参数中，结果还不足以断言超声神经调节是痉挛性脑瘫中痉挛的一种可行治疗方法，还需要更多的研究。
