Food Engineering Technology Research Center/Key Laboratory of Henan Province, School of Food Science and Technology, Henan University of Technology, Zhengzhou, China.
J Sci Food Agric. 2025 Jan 15;105(1):276-284. doi: 10.1002/jsfa.13826. Epub 2024 Aug 21.
Saccharomyces cerevisiae is a fungus widely used in the food industry and biofuel industry, whereas it is usually exposed to high sugar stress during the fermentation process. Ca is a key second messenger of the cell, it can regulate cell metabolism. The present study investigated the effect of the Ca signal on the activity of key enzymes of carbon metabolism and related gene expression in yeast under high sugar fermentation.
The expression of genes encoding hexokinase was up-regulated in the high sugar environment, the activity of hexokinase was increased, glucose transmembrane transport capacity was enhanced, the ability of glucose to enter into glycolytic metabolism was increased, and the expression of genes related to pentose phosphate metabolism, glycerol metabolism and trehalose metabolism was up-regulated in the high glucose with Ca group.
Ca signal regulates the cellular metabolism of glycerol and trehalose and optimizes the allocation of carbon flow by regulating the key enzymes and related gene expression to enhance the resistance of yeast to high sugar stress. © 2024 Society of Chemical Industry.
酿酒酵母是一种广泛应用于食品工业和生物燃料工业的真菌，然而在发酵过程中通常会暴露于高糖应激下。Ca 是细胞的关键第二信使，能够调节细胞代谢。本研究探讨了在高糖发酵条件下 Ca 信号对酵母中碳代谢关键酶活性及相关基因表达的影响。
在高糖环境中，编码己糖激酶的基因表达上调，己糖激酶活性增加，葡萄糖跨膜转运能力增强，葡萄糖进入糖酵解代谢的能力提高，并且与戊糖磷酸代谢、甘油代谢和海藻糖代谢相关的基因表达上调。
Ca 信号通过调节关键酶和相关基因表达来调节甘油和海藻糖的细胞代谢，优化碳流的分配，从而增强酵母对高糖应激的抗性。© 2024 化学工业协会。
