Ministry of Education Engineering Research Center of Starch and Protein Processing, Guangdong Province Key Laboratory for Green Processing of Natural Products and Product Safety, School of Food Science and Engineering, South China University of Technology, Guangzhou 510640, China.
Ministry of Education Engineering Research Center of Starch and Protein Processing, Guangdong Province Key Laboratory for Green Processing of Natural Products and Product Safety, School of Food Science and Engineering, South China University of Technology, Guangzhou 510640, China; Institute of Modern Industrial Technology, South China University of Technology, Zhongshan 528400, China.
Food Chem. 2024 Dec 15;461:140852. doi: 10.1016/j.foodchem.2024.140852. Epub 2024 Aug 12.
Foodborne heterocyclic aromatic amines (HAAs) are potent mutagens and carcinogens, posing significant health risks. Existing enrichment methods for HAAs need better adsorption selectivity and capacity for daily exposure assessment. This study hypothesized that introducing carboxylic groups into magnetic covalent organic frameworks (m-COFs) would improve HAAs adsorption by providing additional binding sites. Hence, we prepared a novel magnetic adsorbent, termed as FeO@DOPA-TpPa-(COOH) capable of enhancing the HAAs detection through magnetic solid-phase extraction (MSPE) coupled with UPLC-MS. This sorbent demonstrated a large specific surface area (130.7 m/g), high magnetic responsivity (21.05 emu/g), and robust stability, with an adsorption capacity (Qm[cal]: 81.82 mg/g) driven by electrostatic, LP - π/C-H - π interactions, and hydrogen bonding. Optimal MSPE conditions provided sensitive detection with a broad linear range (5-500 ng/mL), low limits of detection (0.01-7.01 ng/g), and excellent repeatability. Application to Cantonese mooncake samples showed satisfactory recoveries (62.12%-126.86%). This method offers a more accurate tool for detecting HAAs.
食源杂环胺（HAAs）是强诱变剂和致癌物，对健康构成重大风险。现有的 HAAs 富集方法需要更好的吸附选择性和容量，以进行日常暴露评估。本研究假设在磁性共价有机框架（m-COFs）中引入羧基基团将通过提供额外的结合位点来提高 HAAs 的吸附能力。因此，我们制备了一种新型磁性吸附剂，称为 FeO@DOPA-TpPa-（COOH），能够通过与 UPLC-MS 联用的磁固相萃取（MSPE）来增强 HAAs 的检测。该吸附剂具有较大的比表面积（130.7 m/g）、高磁响应性（21.05 emu/g）和良好的稳定性，其吸附能力（Qm[cal]：81.82 mg/g）是由静电、LP-π/C-H-π 相互作用和氢键驱动的。最佳的 MSPE 条件提供了灵敏的检测，具有较宽的线性范围（5-500 ng/mL）、较低的检测限（0.01-7.01 ng/g）和良好的重复性。将该方法应用于广式月饼样品中，显示出令人满意的回收率（62.12%-126.86%）。该方法为检测 HAAs 提供了更准确的工具。
