Wang Yang, Liu Ying, Chen Ziyan, Liang Shan
Beijing Engineering and Technology Research Center of Food Additives, School of Food and Health, Beijing Technology and Business University (BTBU), Beijing 100048, China.
Molecules. 2025 Apr 10;30(8):1705. doi: 10.3390/molecules30081705.
Heterocyclic aromatic amines (HAAs), known for their mutagenic and carcinogenic potential, are formed during the heating of protein-rich food items. Detecting HAAs swiftly and accurately poses challenges due to complex food matrices and low HAA concentrations. In this study, a simple and efficient magnetic solid-phase extraction (MSPE) strategy was developed for the simultaneous isolation and enrichment of three HAAs such as 2-amino-3,4,8-trimethylimidazo [4,5-f]quinoxaline (4,8-DiMeIQx), 2-amino-3,8-dimethylimidazo [4,5-f]quinoxaline (MeIQx), and 2-amino-3-methylimidazo [4,5-f]quinoline (IQ) in processed meats, employing the magnetic covalent organic framework FeO@MOF-545-AMSA as an adsorbent. It was synthesized via a solvothermal method, with FeO as the magnetic core. Its building blocks are as follows: zirconium (Zr) as the coordination metal ion, tetrakis(4-carboxyphenyl)porphyrin and benzoic acid as organic ligands, and aminomethanesulfonic acid (AMSA). This composite captures targeted HAAs efficiently by exploiting the unique porous MOF-545-AMSA structure, specific metal-ligand coordination, and AMSA's amino and sulfonic acid groups. The quantification of HAAs was achieved through the combination of Ultra-Performance Liquid Chromatography-Tandem Mass Spectrometry (UPLC-MS/MS) and MSPE, demonstrating satisfactory linearity (R ≥ 0.9917), high recovery rates (83.7-111.0%), and low detection limits (0.1-1.0 μg/kg). Moreover, an automated high-throughput detection system was developed using MSPE to assess the presence of HAAs in meat products.
杂环芳香胺（HAAs）因其具有诱变和致癌潜力而闻名，它们在富含蛋白质的食品加热过程中形成。由于食品基质复杂且HAA浓度低，快速准确地检测HAAs具有挑战性。在本研究中，开发了一种简单高效的磁性固相萃取（MSPE）策略，用于同时分离和富集加工肉类中的三种HAAs，即2-氨基-3,4,8-三甲基咪唑[4,5-f]喹喔啉（4,8-二甲基喹喔啉）、2-氨基-3,8-二甲基咪唑[4,5-f]喹喔啉（甲基喹喔啉）和2-氨基-3-甲基咪唑[4,5-f]喹啉（IQ），采用磁性共价有机框架FeO@MOF-545-AMSA作为吸附剂。它是通过溶剂热法合成的，以FeO为磁核。其结构单元如下：锆（Zr）作为配位金属离子，四（4-羧基苯基）卟啉和苯甲酸作为有机配体，以及氨基甲磺酸（AMSA）。该复合材料通过利用独特的多孔MOF-545-AMSA结构、特定的金属-配体配位以及AMSA的氨基和磺酸基团，有效地捕获目标HAAs。通过超高效液相色谱-串联质谱（UPLC-MS/MS）和MSPE相结合实现了HAAs的定量分析，结果表明线性良好（R≥0.9917）、回收率高（83.7-111.0%）且检测限低（0.1-1.0μg/kg）。此外，还开发了一种使用MSPE的自动化高通量检测系统，以评估肉类产品中HAAs的存在情况。
