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蔷薇科拟南芥应答调节因子家族的分子特征及 PbPRR59a 和 PbPRR59b 在开花调控中的功能。

Molecular characterization of PSEUDO RESPONSE REGULATOR family in Rosaceae and function of PbPRR59a and PbPRR59b in flowering regulation.

机构信息

School of Pharmacy, Changzhi Medical College, Changzhi, 046000, China.

Sanya Institute of Nanjing Agricultural University, State Key Laboratory of Crop Genetics & Germplasm Enhancement and Utilization, Jiangsu Key Laboratory for Horticultural Crop Breeding, College of Horticulture, Nanjing Agricultural University, Nanjing, 210095, China.

出版信息

BMC Genomics. 2024 Aug 22;25(1):794. doi: 10.1186/s12864-024-10720-5.

DOI:10.1186/s12864-024-10720-5
PMID:39169310
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11340073/
Abstract

BACKGROUND

PSEUDO RESPONSE REGULATOR (PRR) genes are essential components of circadian clock, playing vital roles in multiple processes including plant growth, flowering and stress response. Nonetheless, little is known about the evolution and function of PRR family in Rosaceae species.

RESULTS

In this study, a total of 43 PRR genes in seven Rosaceae species were identified through comprehensive analysis. The evolutionary relationships were analyzed with phylogenetic tree, duplication events and synteny. PRR genes were classified into three groups (PRR1, PRR5/9, PRR3/7). The expansion of PRR family was mainly derived from dispersed and whole-genome duplication events. Purifying selection was the major force for PRR family evolution. Synteny analysis indicated the existence of multiple orthologous PRR gene pairs between pear and other Rosaceae species. Moreover, the conserved motifs of eight PbPRR proteins supported the phylogenetic relationship. PRR genes showed diverse expression pattern in various tissues of pear (Pyrus bretschneideri). Transcript analysis under 12-h light/ dark cycle and constant light conditions revealed that PRR genes exhibited distinct rhythmic oscillations in pear. PbPRR59a and PbPRR59b highly homologous to AtPRR5 and AtPRR9 were cloned for further functional verification. PbPRR59a and PbPRR59b proteins were localized in the nucleus. The ectopic overexpression of PbPRR59a and PbPRR59b significantly delayed flowering in Arabidopsis transgenic plants by repress the expression of AtGI, AtCO and AtFT under long-day conditions.

CONCLUSIONS

These results provide information for exploring the evolution of PRR genes in plants, and contribute to the subsequent functional studies of PRR genes in pear and other Rosaceae species.

摘要

背景

拟节律调节因子（PRR）基因是生物钟的重要组成部分，在植物生长、开花和应激反应等多个过程中发挥着重要作用。然而，关于 PRR 家族在蔷薇科物种中的进化和功能知之甚少。

结果

本研究通过综合分析，在 7 个蔷薇科物种中鉴定出了 43 个 PRR 基因。利用系统发育树、复制事件和基因同线性分析了它们的进化关系。PRR 基因分为 3 组（PRR1、PRR5/9、PRR3/7）。PRR 家族的扩张主要源于分散和全基因组复制事件。纯化选择是 PRR 家族进化的主要力量。基因同线性分析表明，梨和其他蔷薇科物种之间存在多个直系同源的 PRR 基因对。此外，8 个 PbPRR 蛋白的保守基序支持了系统发育关系。PRR 基因在梨的各种组织中表现出不同的表达模式。12 小时光/暗循环和持续光照条件下的转录分析表明，PRR 基因在梨中表现出明显的节律性振荡。克隆了与 AtPRR5 和 AtPRR9 高度同源的 PbPRR59a 和 PbPRR59b，用于进一步的功能验证。PbPRR59a 和 PbPRR59b 蛋白定位于细胞核。在拟南芥转基因植物中异位过表达 PbPRR59a 和 PbPRR59b 可显著延迟开花，通过在长日照条件下抑制 AtGI、AtCO 和 AtFT 的表达。

结论

这些结果为探索植物中 PRR 基因的进化提供了信息，并为梨和其他蔷薇科物种中 PRR 基因的后续功能研究做出了贡献。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/39c2/11340073/af6ec2009572/12864_2024_10720_Fig9_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/39c2/11340073/d880910d75ac/12864_2024_10720_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/39c2/11340073/b16dc278915e/12864_2024_10720_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/39c2/11340073/eae22a90eb7b/12864_2024_10720_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/39c2/11340073/84d489c4c1c3/12864_2024_10720_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/39c2/11340073/9cfff90cee14/12864_2024_10720_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/39c2/11340073/606afd7a230d/12864_2024_10720_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/39c2/11340073/cddd3fbc0333/12864_2024_10720_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/39c2/11340073/95065f8484ca/12864_2024_10720_Fig8_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/39c2/11340073/af6ec2009572/12864_2024_10720_Fig9_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/39c2/11340073/d880910d75ac/12864_2024_10720_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/39c2/11340073/b16dc278915e/12864_2024_10720_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/39c2/11340073/eae22a90eb7b/12864_2024_10720_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/39c2/11340073/84d489c4c1c3/12864_2024_10720_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/39c2/11340073/9cfff90cee14/12864_2024_10720_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/39c2/11340073/606afd7a230d/12864_2024_10720_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/39c2/11340073/cddd3fbc0333/12864_2024_10720_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/39c2/11340073/95065f8484ca/12864_2024_10720_Fig8_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/39c2/11340073/af6ec2009572/12864_2024_10720_Fig9_HTML.jpg

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