Department of Pharmaceutical Sciences, Pharmacological and Diagnostic Research Center, Faculty of Pharmacy, Al-Ahliyya Amman University, Amman, Jordan.
Al-Kitab University, Kirkuk, Iraq.
J Cosmet Dermatol. 2024 Nov;23(11):3757-3766. doi: 10.1111/jocd.16455. Epub 2024 Aug 21.
The investigations of snail slime and its possible biological activities have been performed recently.
This study aimed to evaluate the properties of Helix aspersa snail slime (HAS), and carotenoids-fortified slime (HASC).
Snails were fed with carrots for 10 days. Slime was collected and extracted using water. Saponins identification, antioxidant, collagenase, and tyrosinase enzyme inhibition activities, carotenoids, total phenols, and flavonoids content, were determined. UHPLC-MS/MS analysis was performed for phytochemical characterization.
Saponins were detected in the HAS extract only. However, HASC was shown to contain a higher carotenoid content than HAS (29.51 ± 0.4 vs. 18.11 ± 0.2 μg/g). similarly, total phenolic and flavonoid content were higher for the the HASC extract compared to the HAS (182.3 ± 5.2 vs. 150.28 ± 3.3 mg/g equivalent to gallic acid), and (77.62 ± 1.2 vs. 14.19 ± 0.9 mg/g equivalent to quercetin). As expected, the HASC extract exhibited higher antioxidant activity compared to the HAS, using DPPH and the ABTS assays (IC = 7.75 ± 0.14 vs. 20.1 ± 0.4 μg/mL), and (IC = 7.6 ± 0.26 vs. 19.57 ± 1.4 μg/mL). UHPLC-MS/MS analysis revealed the presence of several phytocomponents of which, hexadecanoic acid and ascorbic acid, were observed in the HASC extract. Furthermore, HASC extract exhibited superior inhibitory activity compared to HAS against collagenase and tyrosinase enzymes (IC = 8.4 ± 1.19 vs. 15.3 ± 1.12 μg/mL) and (IC = 30.1 ± 0.91 vs. 35 ± 1.3 μg/mL).
These findings highlight the potential of HASC as a valuable ingredient in various pharmaceutical applications, due to their content of various phenolic, antioxidants, carotenoids, hexadecanoic, and ascorbic acids. The latter is well known for its great cosmeceutical applications used for slowing the process of skin aging.
最近对蜗牛黏液及其可能的生物活性进行了研究。
本研究旨在评估海蜗牛黏液（HAS）和富含类胡萝卜素的黏液（HASC）的特性。
蜗牛连续喂养 10 天胡萝卜。使用水收集和提取黏液。鉴定皂甙、抗氧化、胶原酶和酪氨酸酶抑制活性、类胡萝卜素、总酚和类黄酮含量，并进行 UHPLC-MS/MS 分析以进行植物化学成分鉴定。
仅在 HAS 提取物中检测到皂甙。然而，HASC 中的类胡萝卜素含量高于 HAS（29.51 ± 0.4 与 18.11 ± 0.2μg/g）。同样，与 HAS 相比，HASC 提取物中的总酚和类黄酮含量更高（以没食子酸计为 182.3 ± 5.2 与 150.28 ± 3.3μg/g），（以槲皮素计为 77.62 ± 1.2 与 14.19 ± 0.9μg/g）。预期，HASC 提取物在 DPPH 和 ABTS 测定中表现出比 HAS 更高的抗氧化活性（IC=7.75 ± 0.14 与 20.1 ± 0.4μg/mL），（IC=7.6 ± 0.26 与 19.57 ± 1.4μg/mL）。UHPLC-MS/MS 分析表明存在几种植物成分，其中己酸和抗坏血酸存在于 HASC 提取物中。此外，HASC 提取物对胶原酶和酪氨酸酶的抑制活性优于 HAS（IC=8.4 ± 1.19 与 15.3 ± 1.12μg/mL），（IC=30.1 ± 0.91 与 35 ± 1.3μg/mL）。
这些发现强调了 HASC 作为各种药物应用中有价值的成分的潜力，因为它含有各种酚类、抗氧化剂、类胡萝卜素、己酸和抗坏血酸。后者因其在减缓皮肤衰老过程中的良好化妆品应用而广为人知。
