Rahman Nazneen, Rahman Haseebur, Haris Mir, Mahmood Riaz
Department of Biotechnology and Bioinformatics, Kuvempu University, Jnanasahyadri, Shankaraghatta, 577 451, Shimoga Dist., Karnataka, India.
J Tradit Complement Med. 2017 Feb 14;7(4):519-525. doi: 10.1016/j.jtcme.2017.01.005. eCollection 2017 Oct.
is a medicinal plant used in the treatment of external wounds, infected area, ring worms, tumours etc. in traditional system of medicine. The aim of the study was to evaluate the wound healing potentials of leaves hexane (LH) and fruit rind (FW) water extracts and to prove the folkloric claims. The antimicrobial, antioxidant and anti-inflammatory potentials could be important strategies in defining potent wound healing drug. Based on these approaches the current study was designed using incision, excision and dead space wound models with the biochemical, antioxidant enzymes and inflammatory marker analysis. The fruit rind water extract showed highest WBS of 1133 ± 111.4 g. The extracts in excision model retrieved the excised wound i.e. complete healing of wound at day 14. The hydroxyproline content of FW and LH treated dry granuloma tissue was increased to 65.73 ± 3.2 mg/g and 53.66 ± 0.38 mg/g, accompanied by elevations of hexosamine and hexauronic acid with upregulation of GSH, catalase, SOD, peroxidase and the down regulation of the inflammatory marker (NO) and oxidative stress marker (LPO) in wet granulation tissue was documented. Conclusively, both the extracts showed enhanced WBS, rate of wound contraction, skin collagen tissue development, and early epithelisation. Therapeutic wound healing effect was further proven by reduced free radicals and inflammatory makers associated with enhanced antioxidants and connective tissue with histological evidence of more collagen formation. The present research could establish as potential source of effective wound healing drugs.
是一种药用植物，在传统医学体系中用于治疗外部伤口、感染区域、癣、肿瘤等。本研究的目的是评估叶己烷（LH）和果皮（FW）水提取物的伤口愈合潜力，并证实民间说法。抗菌、抗氧化和抗炎潜力可能是确定有效伤口愈合药物的重要策略。基于这些方法，本研究采用切口、切除和死腔伤口模型，并进行生化、抗氧化酶和炎症标志物分析。果皮水提取物显示出最高的伤口断裂强度，为1133±111.4克。在切除模型中，提取物使切除的伤口愈合，即在第14天伤口完全愈合。FW和LH处理的干肉芽组织中的羟脯氨酸含量增加到65.73±3.2毫克/克和53.66±0.38毫克/克，同时己糖胺和己糖醛酸升高，湿肉芽组织中谷胱甘肽、过氧化氢酶、超氧化物歧化酶、过氧化物酶上调，炎症标志物（NO）和氧化应激标志物（LPO）下调。总之，两种提取物均显示出增强的伤口断裂强度、伤口收缩率、皮肤胶原组织发育和早期上皮形成。通过减少与增强的抗氧化剂和结缔组织相关的自由基和炎症标志物，并通过更多胶原蛋白形成的组织学证据，进一步证明了治疗性伤口愈合效果。本研究可以确定其为有效伤口愈合药物的潜在来源。
