Association analysis of leaf aromatic substances in cultivated and weedy types of crop using SSR markers.

In East Asia, particularly South Korea, the two cultivated varieties of are commonly grown. They are clearly distinguished by their aromatic substances and have different uses as leafy vegetables or oil crop. This study was performed for the development of simple sequence repeat (SSR) markers linked to volatile compounds in leaves that show differences between cultivated var. (CF), weedy var. (WF), and weedy var. (WC) of . Fifty SSR primer sets were used to analyze genetic diversity for the 80 accessions of the three types. A total of 276 alleles were detected, with an average of 5.5 alleles per locus. The average genetic diversity values for CF, WF, and WC accessions were 0.402, 0.583, and 0.437, respectively. WF accessions exhibited the highest genetic diversity among the three types of the crop. Phylogenetic tree analysis classified 80 accessions of the three types into four groups, showing 37.2 % genetic similarity. Three types of the crop were clearly distinguished except for outstanding accessions. Through the application of an association analysis involving 50 SSR primer sets and five volatile compounds (perilla aldehyde, perilla ketone, myristicin, dill apiol, (Z,E)-α-farnesene) in the three types of the accessions, we detected 11 significant marker-trait associations duplicated in both Q GLM and Q + K MLM methods. These findings serve as valuable insights for identifying the aromatic substances in plants originating from various regions of South Korea.