Department of Laboratory Medicine, Zhongnan Hospital of Wuhan University, Wuhan University, Wuhan, Hubei 430071, China.
Department of Laboratory Medicine, The First People's Hospital of Tianmen City, Tianmen, Hubei 431700, China.
Int Immunopharmacol. 2024 Nov 15;141:112984. doi: 10.1016/j.intimp.2024.112984. Epub 2024 Aug 21.
The nasopharynx serves as a crucial niche for the microbiome of the upper respiratory tract. However, the association between the intratumoral microbiota and host systemic inflammation and immune status in nasopharyngeal carcinoma (NPC) remain uncertain.
We performed 5R 16S rDNA sequencing on NPC tissue samples, followed by diversity analysis, LEfSe differential analysis, and KEGG functional prediction. The analyses were based on indices such as AISI, SIRI, PAR, PLR, and NAR. Correlation analyses between microbes and these indices were performed to identify microbes associated with inflammation and immune status. Additionally, regression analysis based on tumor TNM stage was performed to identify key microbes linked to tumor progression. The head and neck squamous cell carcinoma (HNSC) transcriptome and the paired HNSC microbiome data from TCGA were utilized to validate the analyses.
The Proteobacteria, Actinobacteria, Firmicutes, and Bacteroidetes were the most enriched phyla in NPC tissues. Microbes within these phyla demonstrated high sensitivity to changes in host systemic inflammation and immune status. Proteobacteria and Firmicutes showed significant differences between inflammation groups. Actinobacteria varied specifically with platelet-related inflammatory indices, and Bacteroidetes genera exhibited significant differences between NAR groups. Corynebacterium and Brevundimonas significantly impacted the T stage of tumors, with a high load of Corynebacterium within tumors associated with a better prognosis CONCLUSION: Our analysis indicates that Proteobacteria play a crucial role in the inflammatory state of NPC, while Bacteroidetes are more sensitive to the tumor immune status.
鼻咽部是上呼吸道微生物组的重要栖息地。然而，鼻咽癌（NPC）肿瘤内微生物群与宿主全身炎症和免疫状态之间的关系尚不清楚。
我们对 NPC 组织样本进行了 5R16S rDNA 测序，随后进行了多样性分析、LEfSe 差异分析和 KEGG 功能预测。分析基于 AISI、SIRI、PAR、PLR 和 NAR 等指标。对微生物与这些指标之间的相关性进行了分析，以确定与炎症和免疫状态相关的微生物。此外，还基于肿瘤 TNM 分期进行了回归分析，以确定与肿瘤进展相关的关键微生物。我们利用 TCGA 中的头颈部鳞状细胞癌（HNSC）转录组和配对 HNSC 微生物组数据来验证分析。
在 NPC 组织中，变形菌门、放线菌门、厚壁菌门和拟杆菌门是最丰富的门。这些门内的微生物对宿主全身炎症和免疫状态的变化具有高度敏感性。变形菌门和厚壁菌门在炎症组之间存在显著差异。放线菌门与血小板相关炎症指标特异性变化，拟杆菌门属在 NAR 组之间存在显著差异。棒状杆菌属和短小芽孢杆菌属显著影响肿瘤的 T 分期，肿瘤内棒状杆菌属负荷较高与预后较好相关。
我们的分析表明，变形菌门在 NPC 的炎症状态中起着关键作用，而拟杆菌门对肿瘤免疫状态更为敏感。
