Department of Biology, College of Science, University of Hafr Al-Batin, Hafr Al-Batin, 39511, Saudi Arabia.
Department of Laboratory Medicine, Faculty of Applied Medical Sciences, Al-Baha University, Al-Baha, Saudi Arabia.
Open Vet J. 2024 Jul;14(7):1596-1606. doi: 10.5455/OVJ.2024.v14.i7.9. Epub 2024 Jul 31.
Cattle and buffaloes can contract cysticercosis, an infection of the muscles brought on by larvae. Despite having a global spread, cysticercosis is more prevalent in impoverished nations due to impaired hygiene standards. It has been discovered that cysticercosis routine visual diagnosis is not very effective, especially in mild infections. Therefore, a more trustworthy test might be used as an alternative in slaughterhouses and epidemiological studies. Biochemical assays are possibly utilized as an alternative to detect cysticercosis inside a topical environment.
Investigating serum biochemical alterations in cattle with cysticercosis was the goal of the current research. As a further method of diagnosis, it was also determined how affected pro-inflammatory cytokines and histopathology.
Blood samples from 42 slaughtered cattle (21 healthy and 21 sick animals) were taken from Assiut abattoir. Using an ELISA and spectrophotometer, respectively, their serum's pro-inflammatory cytokines and biochemical profile were evaluated. These cattle were chosen between March 2023 and February 2024.
A percentage of 4.6% of the 455 cattle examined after being slaughtered had cysticerci infections. All values in the serum biochemistry were considerably different ( < 0.01), whereas the majority of biochemical parameters increased significantly ( < 0.01) in infected animals. In contrast, there was a substantial ( < 0.01) decline in HDL-c, SOD, CAT, and GSH. On the other hand, procytokine inflammatory indices for both TNF-α and IL-1β indicated a substantial increase ( < 0.01) in infected cattle. Additionally, the histological results revealed significant alterations in the tissues of infected livestock.
This has been inferred cysticercosis possesses negative impacts on cattle's plasma biochemical profiles, indicating the field applicability of biochemical measures in outbreaks of bovine cysticercosis. Pro-inflammatory cytokine indices and histological changes could be included as further indicators of cysticercosis in cattle.
牛和水牛可能会感染囊尾蚴病，这是一种由幼虫引起的肌肉感染。尽管囊尾蚴病在全球范围内传播，但由于卫生标准受损，它在贫困国家更为普遍。已经发现，囊尾蚴病的常规视觉诊断效果不是很好，特别是在轻度感染的情况下。因此，在屠宰场和流行病学研究中，可以使用更可靠的测试作为替代方法。生化分析可能被用作检测局部环境中囊尾蚴病的替代方法。
本研究旨在研究患有囊尾蚴病的牛的血清生化变化。作为另一种诊断方法，还确定了受影响的促炎细胞因子和组织病理学变化。
从 Assiut 屠宰场采集了 42 头屠宰牛（21 头健康牛和 21 头患病牛）的血液样本。分别使用 ELISA 和分光光度计评估它们血清中的促炎细胞因子和生化谱。这些牛是在 2023 年 3 月至 2024 年 2 月之间选择的。
屠宰后检查的 455 头牛中有 4.6%患有囊尾蚴感染。血清生化的所有值都有很大差异（<0.01），而感染动物的大多数生化参数显著增加（<0.01）。相反，HDL-c、SOD、CAT 和 GSH 显著下降（<0.01）。另一方面，TNF-α和 IL-1β的前细胞因子炎症指数表明感染牛的显著增加（<0.01）。此外，组织学结果显示感染家畜的组织发生了显著变化。
这表明囊尾蚴病对牛的血浆生化谱有负面影响，表明生化措施在牛囊尾蚴病爆发中的现场适用性。促炎细胞因子指数和组织学变化可以作为牛囊尾蚴病的进一步指标。
