Department of Drug Science and Technology, University of Turin, Via P. Giuria 9, 10125 Turin, Italy.
Department of Chemical Sciences and INSTM Research Unit, University of Catania, Viale Andrea Doria 6, 95125 Catania, Italy.
Bioorg Chem. 2024 Nov;152:107721. doi: 10.1016/j.bioorg.2024.107721. Epub 2024 Aug 14.
Hydrogen peroxide (HO) detection is paramount in biological and clinical domains due to its pivotal role in various physiological and pathological processes. This molecule is a crucial metabolite and effector in cellular redox mechanisms, influencing diverse cellular signaling pathways and bolstering the body's defense mechanisms against infection and oxidative stress. Organic molecule-based electrodes present unique advantages such as operational versatility and scalability, rendering them attractive candidates for sensor development across diverse fields encompassing food safety, healthcare, and environmental monitoring. This study explores the electrochemical properties of a tris(3-hydroxypyridin-4-one) THP, which has been unexplored in electrochemical sensing. Leveraging THP's chelating properties, we aimed to develop an electrochemical probe for hydrogen peroxide detection. Our investigations reveal promising results, with the developed sensor exhibiting a low limit of detection (LOD) of 144 nM, underscoring its potential utility in sensitive and selective HO detection applications. In addition, the new sensor was also tested on fetal bovine serum (FBS) to emphasize future applications on biological matrices. This research signifies a significant stride in advancing electrochemical sensor technologies for hydrogen peroxide detection with several novelties related to the usage of THP, such as high sensitivity and selectivity, performance in biological matrices, repeatability, stability, and reproducibility, economical and practical advantages. This research opens new avenues for enhanced biomedical diagnostics and therapeutic interventions.
过氧化氢（HO）的检测在生物和临床领域至关重要，因为它在各种生理和病理过程中起着关键作用。这种分子是细胞氧化还原机制中的关键代谢物和效应物，影响多种细胞信号通路，并增强身体对感染和氧化应激的防御机制。基于有机分子的电极具有操作多功能性和可扩展性等独特优势，使其成为包括食品安全、医疗保健和环境监测在内的各个领域的传感器开发的有吸引力的候选者。本研究探讨了三（3-羟基吡啶-4-酮）THP 的电化学性质，THP 在电化学传感中尚未得到探索。利用 THP 的螯合特性，我们旨在开发用于过氧化氢检测的电化学探针。我们的研究结果表明，这种新型传感器具有较低的检测限（LOD）为 144 nM，这表明它在灵敏和选择性 HO 检测应用中具有潜在的应用价值。此外，该新型传感器还在胎牛血清（FBS）上进行了测试，以强调其在生物基质中的未来应用。本研究在推进电化学传感器技术用于过氧化氢检测方面取得了重大进展，与 THP 的使用相关的几个新颖性包括高灵敏度和选择性、在生物基质中的性能、重复性、稳定性和重现性、经济和实际优势。这项研究为增强生物医学诊断和治疗干预开辟了新途径。
