Moreno C, Lifely M R, Esdaile J
Infect Immun. 1985 Feb;47(2):527-33. doi: 10.1128/iai.47.2.527-533.1985.
A methodology for preparing outer membrane proteins (type specific) complexed to group B polysaccharide of Neisseria meningitidis is described. These complexes, low in nucleic acid and lipopolysaccharide content, were immunogenic in mice with induction of humoral antigroup B and antiprotein responses. Immunized mice were also protected against challenge with N. meningitidis group B strains of the same or a different type from that used for vaccination. Both immunity and protection were enhanced when the mice received a secondary immunization with the protein-polysaccharide complex. Additional data have shown the capacity of purified B polysaccharide to induce immunological memory, even though it is incapable of inducing a humoral response when given alone.
本文描述了一种制备与脑膜炎奈瑟菌B群多糖复合的外膜蛋白（型特异性）的方法。这些复合物核酸和脂多糖含量低，在小鼠中具有免疫原性，可诱导体液抗B群和抗蛋白反应。免疫的小鼠也受到保护，免受与用于疫苗接种的相同或不同类型的B群脑膜炎奈瑟菌菌株的攻击。当小鼠接受蛋白-多糖复合物的二次免疫时，免疫和保护作用均增强。其他数据表明，纯化的B群多糖具有诱导免疫记忆的能力，尽管单独给予时它不能诱导体液反应。
