Moreno C, Lifely M R, Esdaile J
Infect Immun. 1985 Sep;49(3):587-92. doi: 10.1128/iai.49.3.587-592.1985.
Soluble salts of aluminum were examined for their capacity to complex with purified meningococcal group B polysaccharide. The formation of the complexes resulted, first, in a markedly reduced rate of internal esterification at acid pH and, consequently, prolonged stability of the antigen as measured by its reactivity with antibody at pH 4 and, second, in an increased resistance to neuraminidase. Al3+ complexes of B polysaccharides were tested for immunogenicity in mice and found to be no better than the purified polysaccharide in the Na+ or Ca2+ form. However, when Neisseria meningitidis type 6 protein (outer membrane) complexed to B polysaccharide was tested, a substantial increase in anti-B titers was detected, whereas antiprotein titers remained unchanged. The possibility of using combinations of metal-polysaccharide-outer membrane protein complexes as vaccines for humans is discussed.
对铝的可溶性盐与纯化的B群脑膜炎球菌多糖形成复合物的能力进行了研究。复合物的形成首先导致在酸性pH下内部酯化速率显著降低，因此，通过其在pH 4时与抗体的反应性测定，抗原的稳定性延长；其次，导致对神经氨酸酶的抗性增加。测试了B多糖的Al3+复合物在小鼠中的免疫原性，发现其免疫原性并不比Na+或Ca2+形式的纯化多糖更好。然而，当测试与B多糖复合的6型脑膜炎奈瑟菌蛋白（外膜）时，检测到抗B滴度大幅增加，而抗蛋白滴度保持不变。讨论了使用金属-多糖-外膜蛋白复合物组合作为人类疫苗的可能性。
