Aldalin Hammad K, Alharbi Nada K, Hadi Ameer M, Sharaf Mohamed, Mekky Alsayed E, Ragab Sherif M, Mahmoud Nashaat, Al-Hoshani Nawal, Alwutayd Khairiah M, Abdelnour Sameh A
Department of Medical Support, Al-Karak University College, Al-Balqa Applied University, Al-Karak, Jordan.
Department of Biology, College of Science, Princess Nourah bint Abdulrahman University, Riyadh, Saudi Arabia.
Nat Prod Res. 2024 Aug 24:1-14. doi: 10.1080/14786419.2024.2390608.
The current study evaluates hot water sunflower extracts at 40 °C temperature (AMSE40) for their antibacterial, anti-biofilm, antiviral and anticancer activities. AMSE40 exhibited excellent antioxidant activity with an IC50 of 1.17 mg/mL (ascorbic acid) and was found to be rich in phytochemical compounds such as alkaloids, flavonoids, phenolics, saponins, and tannins, with concentrations of 1.23%, 351.60 mg/g, 152.50 mg/g, 0.98%, and 146.35 mg/g, respectively. AMSE40 showed strong antibacterial and anti-biofilm activity against four multidrug-resistant isolates, comprising , , , and with minimum bactericidal concentrations (25 mg/mL) and minimum inhibitory concentrations (12.5 mg/mL) for all isolates. The AMSE40 (62.5 µg/mL) showed antiviral efficacy against CoxB4 (9.1%) and HSV-1 (34.4%). Additionally, AMSE40 induced DNA fragmentation in liver cell lines, indicating cell death. The cytotoxic concentration of AMSE40 had a mild impact on Vero (283.2 µg) and HepG2 cells (76.4 µg). has the potential to serve as a natural and eco-friendly source for innovative pharmaceutical and medical applications.
本研究评估了40°C温度下的热水向日葵提取物（AMSE40）的抗菌、抗生物膜、抗病毒和抗癌活性。AMSE40表现出优异的抗氧化活性，IC50为1.17mg/mL（抗坏血酸），并被发现富含生物碱、黄酮类化合物、酚类、皂苷和单宁等植物化学化合物，其浓度分别为1.23%、351.60mg/g、152.50mg/g、0.98%和146.35mg/g。AMSE40对四种多重耐药菌株，包括[菌株名称1]、[菌株名称2]、[菌株名称3]和[菌株名称4]表现出强大的抗菌和抗生物膜活性，所有菌株的最小杀菌浓度（25mg/mL）和最小抑菌浓度（12.5mg/mL）。AMSE40（62.5μg/mL）对柯萨奇B4病毒（9.1%）和单纯疱疹病毒1型（34.4%）显示出抗病毒功效。此外，AMSE40诱导肝癌细胞系中的DNA片段化，表明细胞死亡。AMSE40的细胞毒性浓度对Vero细胞（283.2μg）和HepG2细胞（76.4μg）有轻微影响。[AMSE40]有潜力作为创新制药和医疗应用的天然且环保的来源。
