Bhattacharjya Rimki, Kalita Sarojmoni, Dutta Ananya, Basak Dipanwita, Saikia Hemaprobha
Department of Chemistry, Pandu College, Gauhati, Assam, 781012, India.
Department of Chemistry, Gauhati University, Assam, 781014, India.
J Fluoresc. 2025 Jul;35(7):5225-5237. doi: 10.1007/s10895-024-03873-9. Epub 2024 Aug 24.
This work focuses on the synthesis of Bentonite supported nano zero valent bimetallic nanoparticles (B/nZVCu-M NPs) to be utilized for fast and highly sensitive, reversible, fluorescent determination of dopamine (DA) in the presence of dopamine, other biomolecules and ions. The X-ray Photoelectron Spectroscopy(XPS), Powder X-Ray Diffraction(PXRD) and Scanning Electron Microscopy(SEM) revealed the formation of nanoparticles with size ranging from 15 to 20 nm. The composition was revealed by Fourier Transform Infrared(FTIR) Spectoscopy and Energy Dispersive X-Ray (EDX) Analysis. The Limits of Detection(LOD) were noted to be 5.57nM and 6.07nM. The binding of DA is noted to be reversible with respect to EDTA. Furthermore, the developed sensor exhibited good repeatability, satisfactory long-term stability, and was successfully used for the selective detection of dopamine sample with desired recoveries or reversibilities. The main aim of our work is to selectively detect dopamine in presence of its major interferents and biomolecules that are normally present/ co-exist with dopamine in biological systems.
这项工作聚焦于合成膨润土负载的纳米零价双金属纳米颗粒（B/nZVCu-M NPs），用于在多巴胺、其他生物分子和离子存在的情况下快速、高灵敏度、可逆且荧光测定多巴胺（DA）。X射线光电子能谱（XPS）、粉末X射线衍射（PXRD）和扫描电子显微镜（SEM）显示形成了尺寸范围为15至20纳米的纳米颗粒。通过傅里叶变换红外（FTIR）光谱和能量色散X射线（EDX）分析揭示了其组成。检测限（LOD）分别为5.57 nM和6.07 nM。DA与EDTA的结合是可逆的。此外，所开发的传感器具有良好的重复性、令人满意的长期稳定性，并成功用于选择性检测多巴胺样品，回收率或可逆性良好。我们工作的主要目的是在其主要干扰物和生物分子存在的情况下选择性检测多巴胺，这些干扰物和生物分子通常在生物系统中与多巴胺同时存在/共存。
