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用针对犬小孢子菌产生的单克隆抗体对皮肤癣菌分离株进行血清学分析。

Serological analysis of dermatophyte isolates with monoclonal antibodies produced against Microsporum canis.

作者信息

Polonelli L, Morace G

出版信息

J Clin Microbiol. 1985 Jan;21(1):138-9. doi: 10.1128/jcm.21.1.138-139.1985.

DOI:10.1128/jcm.21.1.138-139.1985
PMID:3918075
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC271593/
Abstract

Hybridoma cells produced by fusing myeloma cells with spleen cells from mice immunized with a soluble antigen of Microsporum canis yielded 30 antibody-producing clones. Six of these clones, propagated as ascites tumors in mice, showed two different types of monoclonal antibodies. The type 1 monoclonal antibody reacted with 17 heterologous and 10 homologous dermatophyte antigens. Type 2 monoclonal antibodies were unable to precipitate three antigens from different isolates of M. canis, thus suggesting the occurrence of different serotypes within the species.

摘要

通过将骨髓瘤细胞与用犬小孢子菌可溶性抗原免疫的小鼠脾细胞融合产生的杂交瘤细胞产生了30个产生抗体的克隆。其中6个克隆在小鼠体内作为腹水瘤进行繁殖，显示出两种不同类型的单克隆抗体。1型单克隆抗体与17种异源和10种同源皮肤癣菌抗原发生反应。2型单克隆抗体无法沉淀来自犬小孢子菌不同分离株的三种抗原，因此表明该物种内存在不同的血清型。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7df5/271593/b4814aa6b0cc/jcm00114-0165-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7df5/271593/cf80289bc859/jcm00114-0165-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7df5/271593/b4814aa6b0cc/jcm00114-0165-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7df5/271593/cf80289bc859/jcm00114-0165-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7df5/271593/b4814aa6b0cc/jcm00114-0165-b.jpg

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本文引用的文献

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J Clin Microbiol. 1982 Feb;15(2):204-7. doi: 10.1128/jcm.15.2.204-207.1982.
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Exoantigens of dermatophytes.
Sabouraudia. 1983 Jun;21(2):159-61. doi: 10.1080/00362178385380231.
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Serological differences among the dermatophytes.
Sabouraudia. 1978 Dec;16(4):247-56. doi: 10.1080/00362177885380351.
4
Mycopathologia. 1989 Jul;107(1):17-23. doi: 10.1007/BF00437586.
4
Production and characterization of yeast killer toxin monoclonal antibodies.酵母杀伤毒素单克隆抗体的制备与鉴定
J Clin Microbiol. 1987 Feb;25(2):460-2. doi: 10.1128/jcm.25.2.460-462.1987.
5
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6
Specific and common antigenic determinants of Candida albicans isolates detected by monoclonal antibody.通过单克隆抗体检测白色念珠菌分离株的特异性和共同抗原决定簇。
J Clin Microbiol. 1986 Feb;23(2):366-8. doi: 10.1128/jcm.23.2.366-368.1986.
7
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Improved version of the exoantigen test for identification of Coccidioides immitis and Histoplasma capsulatum cultures.
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