College of Life Science, University of Chinese Academy of Sciences, Beijing 100049, China.
State Key Laboratory of Plant Genomics, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China.
Int J Mol Sci. 2021 Nov 7;22(21):12036. doi: 10.3390/ijms222112036.
Thaxtomin A (TA) is a phytotoxin secreted by that causes common scab in potatoes. However, the mechanism of potato proteomic changes in response to TA is barely known. In this study, the proteomic changes in potato leaves treated with TA were determined using the Isobaric Tags for Relative and Absolute Quantitation (iTRAQ) technique. A total of 693 proteins were considered as differentially expressed proteins (DEPs) following a comparison of leaves treated with TA and sterile water (as a control). Among the identified DEPs, 460 and 233 were upregulated and downregulated, respectively. Based on Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses, many DEPs were found to be involved in defense and stress responses. Most DEPs were grouped in carbohydrate metabolism, amino acid metabolism, energy metabolism, and secondary metabolism including oxidation-reduction process, response to stress, plant-pathogen interaction, and plant hormone signal transduction. In this study, we analyzed the changes in proteins to elucidate the mechanism of potato response to TA, and we provided a molecular basis to further study the interaction between plant and TA. These results also offer the option for potato breeding through analysis of the resistant common scab.
Thaxtomin A（TA）是一种由 分泌的植物毒素，可导致马铃薯普通疮痂病。然而，关于 TA 引起马铃薯蛋白质组变化的机制尚不清楚。在本研究中，采用 Isobaric Tags for Relative and Absolute Quantitation（iTRAQ）技术测定了 TA 处理马铃薯叶片的蛋白质组变化。通过比较 TA 处理和无菌水（作为对照）处理的叶片，共鉴定出 693 种差异表达蛋白（DEPs）。在鉴定出的 DEPs 中，分别有 460 种和 233 种上调和下调。基于基因本体论（GO）和京都基因与基因组百科全书（KEGG）分析，发现许多 DEPs 参与防御和应激反应。大多数 DEPs 被归类为碳水化合物代谢、氨基酸代谢、能量代谢和次生代谢，包括氧化还原过程、应激反应、植物-病原体相互作用和植物激素信号转导。在本研究中，我们分析了蛋白质的变化，以阐明马铃薯对 TA 反应的机制，并为进一步研究植物与 TA 之间的相互作用提供了分子基础。这些结果还为通过分析抗普通疮痂病的马铃薯品种提供了选择。
