Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran; Research Center for Advanced Materials, Faculty of Materials Engineering, Sahand University of Technology, Tabriz, Iran.
Department of Neuroscience and Cognition, Faculty of Advanced Medical Sciences, Tabriz University of Medical Sciences, Tabriz, Iran.
Int J Biol Macromol. 2024 Oct;278(Pt 4):135014. doi: 10.1016/j.ijbiomac.2024.135014. Epub 2024 Aug 22.
Biocompatible scaffolds with high mechanical strengths that contain biodegradable components could boost bone regeneration compared with nondegradable bone repair materials. In this study, porous chitosan (CS)/hydroxyapatite (HA) scaffolds containing mesoporous SiO-HA particles were fabricated through the freeze-drying process. According to field emission scanning electron microscopy (FESEM) results, combining mesoporous SiO-HA particles in CS/HA scaffolds led to a uniform porous structure. It decreased pore sizes from 320 ± 1.1 μm to 145 ± 1.4 μm. Moreover, the compressive strength value of this scaffold was 25 ± 1.2 MPa. The in-vitro approaches exhibited good sarcoma osteogenic cell line (SAOS-2) adhesion, spreading, and proliferation, indicating that the scaffolds provided a suitable environment for cell cultivation. Also, in-vivo analyses in implanted defect sites of rats proved that the CS/HA/mesoporous SiO-HA scaffolds could promote bone regeneration via enhancing osteoconduction and meliorating the expression of osteogenesis gene to 19.31 (about 5-fold higher compared to the control group) by exposing them to the bone-like precursors. Further, this scaffold's new bone formation percentage was equal to 90 % after 21 days post-surgery. Therefore, incorporating mesoporous SiO-HA particles into CS/HA scaffolds can suggest a new future tissue engineering and regeneration strategy.
具有高机械强度和可生物降解成分的生物相容性支架可促进骨再生，优于不可降解的骨修复材料。本研究通过冷冻干燥工艺制备了含有介孔 SiO2-HA 颗粒的多孔壳聚糖 (CS)/羟基磷灰石 (HA) 支架。根据场发射扫描电子显微镜 (FESEM) 结果，在 CS/HA 支架中结合介孔 SiO2-HA 颗粒可得到均匀的多孔结构。这将孔径从 320±1.1μm 减小到 145±1.4μm。此外，该支架的压缩强度值为 25±1.2MPa。体外方法显示出良好的骨肉瘤成骨细胞系（SAOS-2）黏附、铺展和增殖能力，表明支架为细胞培养提供了合适的环境。此外，在大鼠植入缺陷部位的体内分析表明，CS/HA/介孔 SiO2-HA 支架可通过增强成骨作用和改善成骨基因的表达（与对照组相比提高 19.31 倍）来促进骨再生，从而使骨样前体暴露。进一步的，该支架在手术后 21 天的新骨形成率达到 90%。因此，将介孔 SiO2-HA 颗粒掺入 CS/HA 支架中可能为组织工程和再生策略提供新的思路。
