Institute of Biomaterials, University of Erlangen-Nuremberg, Cauerstraße 6, 91058 Erlangen, Germany.
Department of Cariology, Restorative Sciences, and Endodontics, School of Dentistry, University of Michigan, Ann Arbor, MI, USA.
Dent Mater. 2024 Nov;40(11):1832-1842. doi: 10.1016/j.dental.2024.08.006. Epub 2024 Aug 24.
Calcium silicate cements (CSCs) are often used in endodontics despite some limitations related to their physical properties and antibacterial efficacy. This study aimed to develop and demonstrate the viability of a series of CSCs that were produced by sol-gel method and further modified with mesoporous bioactive glass nanoparticles (MBGNs) and collagen, for endodontic therapy.
Calcium silicate (CS) particles and MBGNs were synthesized by the sol-gel method, and their elemental, molecular, and physical microstructure was characterized. Three CSCs were developed by mixing the CS with distilled water (CS+HO), 10 mg/mL collagen solution (CS+colHO), and MBGNs (10 %) (CSmbgn+colHO). The mixing (MT) and setting (ST) times of the CSCs were determined, while the setting reaction was monitored in real-time. Antibacterial efficacy against Enterococcus faecalis (E. faecalis) and regenerative potential on dental pulp stem cells (DPSCs) were also analyzed.
The CS+HO displayed a ST comparable to commercial products, while CSmbgn+colHO achieved the longest MT of 68 s and the shortest ST of 8 min. All the experimental CSCs inhibited the growth of E. faecalis. Additionally, compared to the control group, CSCs supported cell proliferation and spreading and mineralized matrix production, regardless of their composition.
Tested CSCs presented potential as candidates for pulp therapy procedures. Future research should investigate the pulp regeneration mechanisms alongside rigorous antibacterial evaluations, preferably with multi-organism biofilms, executed over extended periods.
尽管硅酸钙水泥（CSC）在物理性能和抗菌效果方面存在一些局限性，但仍常被用于牙髓学领域。本研究旨在开发并证明一系列通过溶胶-凝胶法制备并进一步用介孔生物活性玻璃纳米颗粒（MBGNs）和胶原修饰的 CSC 用于牙髓治疗的可行性。
通过溶胶-凝胶法合成硅酸钙（CS）颗粒和 MBGNs，并对其元素、分子和物理微观结构进行了表征。通过将 CS 与蒸馏水（CS+HO）、10mg/mL 胶原溶液（CS+colHO）和 10%（质量分数）MBGNs（CSmbgn+colHO）混合，开发了三种 CSC。测定了 CSC 的混合（MT）和凝固（ST）时间，并实时监测凝固反应。还分析了它们对粪肠球菌（E. faecalis）的抗菌效果和对牙髓干细胞（DPSCs）的再生潜力。
CS+HO 的 ST 与商业产品相当，而 CSmbgn+colHO 的 MT 最长（68s），ST 最短（8min）。所有实验性 CSC 均抑制了粪肠球菌的生长。此外，与对照组相比，CSC 支持细胞增殖、铺展以及矿化基质的产生，而与它们的组成无关。
经测试的 CSC 具有作为牙髓治疗程序候选物的潜力。未来的研究应在更长时间内使用多生物体生物膜进行严格的抗菌评估，同时调查牙髓再生机制。
