Jiangsu Key Laboratory of Oral Diseases, Nanjing Medical University, Nanjing, People's Republic of China.
Department of Endodontics, The Affiliated Stomatological Hospital of Nanjing Medical University, Nanjing, People's Republic of China.
Int J Nanomedicine. 2020 Jun 3;15:3921-3936. doi: 10.2147/IJN.S244686. eCollection 2020.
Mesoporous calcium-silicate nanoparticles (MCSNs) have good prospects in the medical field due to their great physicochemical characteristics, antibacterial activity and drug delivery capacity. This study was to analyze the antibiofilm activity and mechanisms of silver (Ag) and zinc (Zn) incorporated MCSNs (Ag/Zn-MCSNs) with different percentages of Ag and Zn.
Ag/Zn(1:9, molar ratio)-MCSNs and Ag/Zn(9:1, molar ratio)-MCSNs were prepared and characterized. Endocytosis of nanoparticles by () treated with Ag/Zn-MCSNs was observed using TEM to explore the antibacterial mechanisms. The antibiofilm activity of Ag/Zn-MCSNs with different ratios of Ag and Zn was tested by biofilm model in human roots. The human roots pretreated by different Ag/Zn-MCSNs were cultured with . Then, SEM and CLSM were used to observe the survival of on the root canal wall. Cytotoxicity of the nanoparticles was tested by CCK8 kits.
The Ag/Zn-MCSNs release Ag and destroy the cell membranes to kill bacteria. The MCSNs containing Ag showed antibacterial activity against biofilms in different degrees, and they can adhere to dentin surfaces to get a continuous antibacterial effect. However, MTA, MCSNs and Zn-MCSNs could not disrupt the bacterial biofilms obviously. MCSNs, Ag/Zn(1:1, molar ratio)-MCSNs and Ag/Zn(1:9)-MCSNs showed no obvious cytotoxicity, while Ag-MCSNs and Ag/Zn(9:1)-MCSNs showed cytotoxicity. Zn-MCSNs can slightly promote cell proliferation.
Ag/Zn-MCSNs have good antibiofilm activity. They might achieve an appropriate balance between the antibacterial activity and cytotoxicity by adjusting the ratio of Ag and Zn. Ag/Zn-MCSNs are expected to be a new type of root canal disinfectant or sealer for root canal treatment.
介孔硅酸钙纳米颗粒（MCSNs）具有良好的物理化学特性、抗菌活性和药物输送能力，在医学领域具有广阔的前景。本研究旨在分析不同比例银（Ag）和锌（Zn）掺入的 MCSNs（Ag/Zn-MCSNs）的抗生物膜活性和机制。
制备并表征了 Ag/Zn（1:9，摩尔比）-MCSNs 和 Ag/Zn（9:1，摩尔比）-MCSNs。通过 TEM 观察用 Ag/Zn-MCSNs 处理的（）对纳米颗粒的内吞作用，以探讨抗菌机制。采用人牙根生物膜模型，检测不同比例 Ag 和 Zn 的 Ag/Zn-MCSNs 的抗生物膜活性。用不同比例的 Ag/Zn-MCSNs 预处理人牙根，然后用培养。然后，用 SEM 和 CLSM 观察根管壁上的 存活情况。用 CCK8 试剂盒检测纳米颗粒的细胞毒性。
Ag/Zn-MCSNs 释放 Ag 并破坏细胞膜杀死细菌。含 Ag 的 MCSNs 对 生物膜表现出不同程度的抗菌活性，可黏附在牙本质表面，获得持续的抗菌效果。然而，MTA、MCSNs 和 Zn-MCSNs 并不能明显破坏细菌生物膜。MCSNs、Ag/Zn（1:1，摩尔比）-MCSNs 和 Ag/Zn（1:9）-MCSNs 无明显细胞毒性，而 Ag-MCSNs 和 Ag/Zn（9:1）-MCSNs 则表现出细胞毒性。Zn-MCSNs 可轻微促进细胞增殖。
Ag/Zn-MCSNs 具有良好的抗生物膜活性。通过调整 Ag 和 Zn 的比例，可以在抗菌活性和细胞毒性之间达到适当的平衡。Ag/Zn-MCSNs 有望成为根管治疗的新型根管消毒剂或封闭剂。
