Stemwedel Katharina, Haase Nadin, Christ Simon, Bogdanova Natalia V, Rudorf Sophia
Leibniz University Hannover, Institute of Cell Biology and Biophysics, Hannover, 30167, Germany.
Hannover Medical School, Radiation Oncology, Hannover, 30625, Germany.
NAR Genom Bioinform. 2024 Aug 24;6(3):lqae110. doi: 10.1093/nargab/lqae110. eCollection 2024 Sep.
Ionizing radiation (IR) in high doses is generally lethal to most organisms. Investigating mechanisms of radiation resistance is crucial for gaining insights into the underlying cellular responses and understanding the damaging effects of IR. In this study, we conducted a comprehensive analysis of sequencing data from an evolutionary experiment aimed at understanding the genetic adaptations to ionizing radiation in . By including previously neglected synonymous mutations, we identified the c.294T > G variant, which emerged in all 17 examined isolates across four subpopulations. The identified variant is a synonymous mutation affecting the 30S ribosomal protein S8, and consistently exhibited high detection and low allele frequencies in all subpopulations. This variant, along with two additional variants, potentially influences translational control of the ribosomal operon. The early emergence and stability of these variants suggest their role in adapting to environmental stress, possibly contributing to radiation resistance. Our findings shed light on the dynamics of ribosomal variants during the evolutionary process and their potential role in stress adaptation, providing valuable implications for understanding clinical radiation sensitivity and improving radiotherapy.
高剂量的电离辐射（IR）通常对大多数生物是致命的。研究辐射抗性机制对于深入了解潜在的细胞反应以及理解IR的破坏作用至关重要。在本研究中，我们对一项进化实验的测序数据进行了全面分析，该实验旨在了解[具体对象]对电离辐射的遗传适应性。通过纳入先前被忽视的同义突变，我们鉴定出了c.294T>G变体，该变体在四个亚群的所有17个检测分离株中均出现。所鉴定的变体是一个影响30S核糖体蛋白S8的同义突变，并且在所有亚群中始终表现出高检出率和低等位基因频率。这个变体，连同另外两个变体，可能影响核糖体操纵子的翻译控制。这些变体的早期出现和稳定性表明它们在适应环境压力中的作用，可能有助于辐射抗性。我们的发现揭示了进化过程中核糖体变体的动态变化及其在应激适应中的潜在作用，为理解临床辐射敏感性和改善放射治疗提供了有价值的启示。
