Cerretti D P, Dean D, Davis G R, Bedwell D M, Nomura M
Nucleic Acids Res. 1983 May 11;11(9):2599-616. doi: 10.1093/nar/11.9.2599.
The genes encoding the 52 ribosomal proteins (r-proteins) of Escherichia coli are organized into approximately 19 operons scattered throughout the chromosome. One of these, the spc operon, contains the genes for ten ribosomal proteins: L14, L24, L5, S14, S8, L6, L18, S5, L30 and L15 (rp1N, rp1X, rp1E, rpsN, rpsH, rp1F, rp1R, rpsE, rpmD, and rp1O). We now report the entire 5.9 kb nucleotide sequence of the spc operon. DNA sequence analysis has confirmed the genetic organization and refined the amino acid sequence of the ten r-proteins in this operon. It has also revealed the presence of two open reading frames past the last known gene (L15) of the spc operon. One of these corresponds to a gene (pr1A or secY) which recently has been shown by others to be involved in protein export. In addition, S1 mapping experiments indicate that a significant proportion of transcription initiated from the spc operon continues not only into the two putative genes, but also without termination into the downstream alpha r-protein operon.
编码大肠杆菌52种核糖体蛋白（r - 蛋白）的基因被组织成大约19个操纵子，分散在整个染色体上。其中之一，即spc操纵子，包含十个核糖体蛋白的基因：L14、L24、L5、S14、S8、L6、L18、S5、L30和L15（rp1N、rp1X、rp1E、rpsN、rpsH、rp1F、rp1R、rpsE、rpmD和rp1O）。我们现在报告spc操纵子的完整5.9 kb核苷酸序列。DNA序列分析证实了该操纵子的基因组织，并完善了其中十个r - 蛋白的氨基酸序列。它还揭示了在spc操纵子最后一个已知基因（L15）之后存在两个开放阅读框。其中一个对应于一个基因（pr1A或secY），最近其他人已表明该基因参与蛋白质输出。此外S1图谱实验表明，从spc操纵子起始的相当一部分转录不仅持续进入这两个推定的基因，而且无终止地持续进入下游的α r - 蛋白操纵子。
