Fares Reham, Elasmer Shimaa M, A Abeer Khalefa, Shaker Olfat G, El-Tahlawi Samar M, Sabri Ahmed, Yaseen Sara M
Department of Medical Biochemistry and Molecular Biology Faculty of Medicine Fayoum University, Fayoum, Egypt.
Department of Clinical and Chemical Pathology Faculty of Medicine Fayoum University, Fayoum, Egypt.
Dermatol Res Pract. 2024 Aug 16;2024:3163839. doi: 10.1155/2024/3163839. eCollection 2024.
Mycosis fungoides (MF) is a type of cutaneous T-cell lymphoma where red rash exists on the skin. Understanding the role of miRNAs and ncRNAs in p53-response has become an open discussion, as they can regulate p53 or its downstream targets, and ncRNAs themselves.
To evaluate the serum levels of NEAT-1, miR-34a, and p53 in MF patients and its relation to healthy controls to indicate whether it has a potential role in the pathogenesis of the disease. . This prospective case-control study was carried out on 75 subjects subdivided into two groups, 35 MF patients (stages 1 and II) and 40 matched healthy controls. Their clinical investigations and serum biomarkers (NEAT-1, miR-34a, and p53) were measured.
There were significant elevations in the expression levels of both NEAT-1 (5.10 ± 1.16) and p53 (277.28 ± 62.02) in the serum of MF patients in comparison with controls (1.01 ± 0.031) and (194.29 ± 16.039), respectively, while the level of miR-34a tends to decrease in MF patients (0.24 ± 0.15). There are no significant difference between MF stages and the level of miR-34a, while in NEAT-1 and p53, there are significant differences with value <0.05 between the stages and the biomarkers. There is a positive correlation between the %BSA and miR-34a and a slightly positive correlation between NEAT-1 and P53 with ( = 0.353, =0.037) and ( = 0112, =0.05), respectively. There were also negative correlations between disease duration and NEAT-1 with ( = -0.341, =0.045) and between B2 microglobulin level and p53 ( = -0.373, =0.027).
The combination of miR-34a, NEAT-1, and p53 may be considered as potential biomarkers that play an active role in the disease process of MF for helping in its early diagnosis and stage identification as well.
蕈样肉芽肿（MF）是一种皮肤T细胞淋巴瘤，皮肤上会出现红色皮疹。由于微小RNA（miRNA）和非编码RNA（ncRNA）可以调节p53或其下游靶点以及ncRNA自身，因此了解它们在p53反应中的作用已成为一个开放性的讨论话题。
评估MF患者血清中核富集转录本1（NEAT-1）、miR-34a和p53的水平及其与健康对照的关系，以表明它们在该疾病发病机制中是否具有潜在作用。本前瞻性病例对照研究对75名受试者进行，分为两组，35例MF患者（I期和II期）和40例匹配的健康对照。对他们进行了临床检查并检测了血清生物标志物（NEAT-1、miR-34a和p53）。
与对照组相比，MF患者血清中NEAT-1（5.10±1.16）和p53（277.28±62.02）的表达水平显著升高，对照组中NEAT-1和p53水平分别为（1.01±0.031）和（194.29±16.039），而MF患者中miR-34a水平趋于降低（0.24±0.15）。MF各期与miR-34a水平之间无显著差异，而在NEAT-1和p53方面，各期与生物标志物之间存在显著差异，P值<0.05。体表面积百分比（%BSA）与miR-34a呈正相关，NEAT-1与P53呈轻微正相关，相关系数分别为（r = 0.353，P = 0.037）和（r = 0.112，P = 0.05）。疾病持续时间与NEAT-1之间也呈负相关（r = -0.341，P = 0.045），β2微球蛋白水平与p53之间也呈负相关（r = -0.373，P = 0.027）。
miR-34a、NEAT-1和p53的组合可被视为潜在的生物标志物，它们在MF疾病过程中发挥积极作用，有助于早期诊断和分期识别。
