Prieto Daniel, Egger Boris, Cantera Rafael
Departamento de Biología del Neurodesarrollo, Instituto de Investigaciones Biológicas Clemente Estable, Montevideo, Uruguay.
Departamento de Neurofisiología Celular y Molecular, Instituto de Investigaciones Biológicas Clemente Estable, Montevideo, Uruguay.
MicroPubl Biol. 2024 Aug 11;2024. doi: 10.17912/micropub.biology.001252. eCollection 2024.
Hypoxia-induced proliferation of neural stem cells has a crucial role in brain development. In the brain of , the optic lobe exhibits progressive hypoxia during larval development. Here, we investigate an alternative oxygen-sensing mechanism within this brain compartment, distinct from the canonical hypoxia signaling pathway mediated by HIF. Using genetic tools, immunostaining, and confocal microscopy, we demonstrate that the loss of the atypical soluble guanylyl cyclase (asGC) subunit , or the ectopic expression of in neural stem cells leads to increased optic lobe volume. We propose the existence of a link between cGMP signaling and neurogenesis in the developing brain.
缺氧诱导的神经干细胞增殖在大脑发育中起着关键作用。在[未提及的生物名称]的大脑中，视叶在幼虫发育过程中呈现渐进性缺氧。在此，我们研究了这个脑区中一种不同于由低氧诱导因子介导的经典缺氧信号通路的替代性氧感知机制。通过基因工具、免疫染色和共聚焦显微镜，我们证明非典型可溶性鸟苷酸环化酶（asGC）亚基[未提及的亚基名称]的缺失，或神经干细胞中[未提及的物质]的异位表达会导致视叶体积增加。我们提出在发育中的大脑中，环磷酸鸟苷（cGMP）信号与神经发生之间存在联系。
