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生物信息学分析及鉴定结直肠癌中铜死亡相关长非编码 RNA

Bioinformatics analysis and identification of cuproptosis-related long non-coding RNAs in colorectal cancer.

机构信息

Department of Anxi County Hospital, Quanzhou, PR China.

出版信息

J Int Med Res. 2024 Aug;52(8):3000605241274563. doi: 10.1177/03000605241274563.

DOI:10.1177/03000605241274563
PMID:39188141
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11350552/
Abstract

OBJECTIVE

Identifying precise biomarkers for colorectal cancer (CRC) detection and management remains challenging. Here, we developed an innovative prognostic model for CRC using cuproptosis-related long non-coding RNAs (lncRNAs).

METHODS

In this retrospective study, CRC patient transcriptomic and clinical data were sourced from The Cancer Genome Atlas database. Cuproptosis-related lncRNAs were identified and used to develop a prognostic model, which helped categorize patients into high- and low-risk groups. The model was validated through survival analysis, risk curves, independent prognostic analysis, receiver operating characteristic curve analysis, decision curves, and nomograms. In addition, we performed various immune-related analyses. LncRNA expression levels were examined in normal human colorectal epithelial cells (FHC) and CRC cells (HCT-116) using quantitative polymerase chain reaction (qPCR).

RESULTS

Six cuproptosis-related lncRNAs were identified: ZKSCAN2-DT, AL161729.4, AC016394.1, AC007128.2, AL137782.1, and AC099850.3. The prognostic model distinguished between high-/low-risk populations, demonstrating excellent predictive ability for survival outcomes. Immunocorrelation analysis showed significant differences in immune cell infiltration and functions, immune checkpoint expression, and mA methylation-related genes. The qPCR results showed significant upregulation of ZKSCAN2-DT, AL161729.4, AC016394.1, AC007128.2 in HCT-116 cells, while AL137782.1 and AC099850.3 expression patterns were significantly downregulated.

CONCLUSION

Cuproptosis-related lncRNAs can potentially serve as reliable diagnostic and prognostic biomarkers for CRC.

摘要

目的

寻找用于结直肠癌（CRC）检测和管理的精确生物标志物仍然具有挑战性。在这里，我们使用铜死亡相关长非编码 RNA（lncRNA）开发了一种用于 CRC 的创新预后模型。

方法

在这项回顾性研究中，CRC 患者的转录组和临床数据来自癌症基因组图谱（TCGA）数据库。鉴定铜死亡相关 lncRNA，并用于开发预后模型，该模型帮助将患者分为高风险和低风险组。通过生存分析、风险曲线、独立预后分析、受试者工作特征曲线分析、决策曲线和列线图对模型进行验证。此外，我们还进行了各种免疫相关分析。使用实时定量聚合酶链反应（qPCR）检测正常人类结直肠上皮细胞（FHC）和 CRC 细胞（HCT-116）中的 lncRNA 表达水平。

结果

确定了六个铜死亡相关 lncRNA：ZKSCAN2-DT、AL161729.4、AC016394.1、AC007128.2、AL137782.1 和 AC099850.3。预后模型区分了高/低风险人群，对生存结果具有出色的预测能力。免疫相关性分析显示，免疫细胞浸润和功能、免疫检查点表达以及 mA 甲基化相关基因存在显著差异。qPCR 结果显示，HCT-116 细胞中 ZKSCAN2-DT、AL161729.4、AC016394.1、AC007128.2 的表达显著上调，而 AL137782.1 和 AC099850.3 的表达模式则显著下调。

结论

铜死亡相关 lncRNA 可能成为 CRC 可靠的诊断和预后生物标志物。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eef7/11350552/aab838f74adc/10.1177_03000605241274563-fig6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eef7/11350552/f142511da43d/10.1177_03000605241274563-fig1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eef7/11350552/b13dbb2173fb/10.1177_03000605241274563-fig2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eef7/11350552/26d4cff13cc7/10.1177_03000605241274563-fig3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eef7/11350552/f417782f706a/10.1177_03000605241274563-fig4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eef7/11350552/5f3ed4c222c2/10.1177_03000605241274563-fig5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eef7/11350552/aab838f74adc/10.1177_03000605241274563-fig6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eef7/11350552/f142511da43d/10.1177_03000605241274563-fig1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eef7/11350552/b13dbb2173fb/10.1177_03000605241274563-fig2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eef7/11350552/26d4cff13cc7/10.1177_03000605241274563-fig3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eef7/11350552/f417782f706a/10.1177_03000605241274563-fig4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eef7/11350552/5f3ed4c222c2/10.1177_03000605241274563-fig5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eef7/11350552/aab838f74adc/10.1177_03000605241274563-fig6.jpg

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本文引用的文献

1
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Biomedicines. 2024 Apr 23;12(5):932. doi: 10.3390/biomedicines12050932.
2
The construction of a novel ferroptosis-related lncRNA model to predict prognosis in colorectal cancer patients.构建新型铁死亡相关 lncRNA 模型预测结直肠癌患者预后
Medicine (Baltimore). 2023 Mar 10;102(10):e33114. doi: 10.1097/MD.0000000000033114.
3
Copper homeostasis and cuproptosis in health and disease.铜稳态和铜死亡在健康和疾病中的作用。
Signal Transduct Target Ther. 2022 Nov 23;7(1):378. doi: 10.1038/s41392-022-01229-y.
4
Exploring the role of m6A modification in cancer.探讨 m6A 修饰在癌症中的作用。
Proteomics. 2023 Jul;23(13-14):e2200208. doi: 10.1002/pmic.202200208. Epub 2022 Nov 17.
5
WTAP Mediated the N6-methyladenosine Modification of PDK4 to Regulate the Malignant Behaviors of Colorectal Cancer Cells and I.WTAP 通过调控 PDK4 的 N6-甲基腺苷修饰来调节结直肠癌细胞的恶性行为
Curr Med Chem. 2023;30(29):3368-3381. doi: 10.2174/0929867329666220922102949.
6
Surgery for T4 Colorectal Cancer in Older Patients: Determinants of Outcomes.老年患者T4期结直肠癌手术：预后的决定因素
J Pers Med. 2022 Sep 19;12(9):1534. doi: 10.3390/jpm12091534.
7
Signaling by LncRNAs: Structure, Cellular Homeostasis, and Disease Pathology.长链非编码 RNA 的信号转导：结构、细胞稳态和疾病病理。
Cells. 2022 Aug 13;11(16):2517. doi: 10.3390/cells11162517.
8
Cuproptosis-Related lncRNAs are Biomarkers of Prognosis and Immune Microenvironment in Head and Neck Squamous Cell Carcinoma.铜死亡相关长链非编码RNA是头颈部鳞状细胞癌预后和免疫微环境的生物标志物。
Front Genet. 2022 Jul 22;13:947551. doi: 10.3389/fgene.2022.947551. eCollection 2022.
9
Cuproptosis: copper-induced regulated cell death.铜死亡：铜诱导的程序性细胞死亡。
Sci China Life Sci. 2022 Aug;65(8):1680-1682. doi: 10.1007/s11427-022-2106-6. Epub 2022 Apr 24.
10
Integrated Analysis of a Ferroptosis-Related LncRNA Signature for Evaluating the Prognosis of Patients with Colorectal Cancer.基于铁死亡相关长链非编码 RNA signature 的综合分析评估结直肠癌患者的预后
Genes (Basel). 2022 Jun 19;13(6):1094. doi: 10.3390/genes13061094.