Departamento de Bioquímica e Biofísica, Instituto de Ciências da Saúde, Universidade Federal da Bahia, Salvador 40170-110, Bahia, Brazil.
Maternidade Climério de Oliveira (MCO/EBSERH), Universidade Federal da Bahia, Salvador 40055-150, Bahia, Brazil.
Med Sci (Basel). 2024 Aug 16;12(3):41. doi: 10.3390/medsci12030041.
The progression of chronic obstructive pulmonary disease (COPD) is characterized by functional changes in the airways. The lung-gut axis and gut microbiota (GM) have been linked to the pathophysiology of airway diseases. Regarding COPD, studies have shown that GM alterations could be related the stages of this disease. However, the relationship between GM and clinical, biochemical and immunological parameters in patients with COPD are not well understood. The aim of this study was to compare the relative abundance of specific groups of beneficial gut bacteria between COPD patients and healthy controls (CTLs) in order to evaluate relationships with metabolic and inflammatory markers in COPD.
We included 16 stable COPD patients and 16 healthy volunteer CTLs. The relative abundances of spp. (Bf) and (Akk) bacteria and the Bacteroidetes and Firmicutes phyla were assessed by qPCR. Pulmonary function was evaluated by spirometry, biochemical parameters by colorimetric methods and plasma cytokine levels by cytometric bead array analysis.
The Firmicutes/Bacteroides ratio was related to emergency hospital visits and six-minute walk test (6MWT) results. Furthermore, the relative abundance of Bf was associated with plasma concentrations of glucose, triglycerides, HDL-C and IL-10. In addition, Firmicutes levels and the Firmicutes/Bacteroidetes ratio were associated with the IL-12/IL-10 ratio, while Akk abundance was linked to IL-12 levels.
The present findings suggest that the abundance of beneficial bacteria in the GM could influence clinical presentation and immunoregulation in COPD.
本研究旨在比较 COPD 患者和健康对照（CTL）之间特定有益肠道细菌群体的相对丰度，以评估其与 COPD 中代谢和炎症标志物的关系。
我们纳入了 16 例稳定期 COPD 患者和 16 例健康志愿者 CTL。通过 qPCR 评估 spp.（Bf）和 （Akk）细菌以及拟杆菌门和厚壁菌门的相对丰度。通过肺量计评估肺功能，通过比色法评估生化参数，通过流式细胞术检测分析评估血浆细胞因子水平。
Firmicutes/Bacteroides 比值与急诊就诊和 6 分钟步行试验（6MWT）结果相关。此外，Bf 的相对丰度与血浆葡萄糖、甘油三酯、HDL-C 和 IL-10 浓度相关。此外，Firmicutes 水平和 Firmicutes/Bacteroidetes 比值与 IL-12/IL-10 比值相关，而 Akk 丰度与 IL-12 水平相关。
本研究结果表明，GM 中有益细菌的丰度可能影响 COPD 的临床表现和免疫调节。
