Institute of Chemistry, St. Petersburg State University, St. Petersburg, Russia.
J Sep Sci. 2024 Aug;47(16):e2400352. doi: 10.1002/jssc.202400352.
This review provides an overview of recent works focusing on the determination of amino acids (AAs) and peptides using capillary electrophoresis with contactless conductivity detection and ultraviolet (UV) detection, which is the most widespread detection in capillary electromigration techniques, without pre-capillary derivatization. Available options for the UV detection of these analytes, such as indirect detection, complexation with transition metal ions, and in-capillary derivatization are described. Developments in the field of direct detection of UV-absorbing AAs and peptides as well as progress in chiral separation are described. A separate section is dedicated to using on-line sample preconcentration methods combined with capillary electrophoresis-UV.
本综述概述了近年来使用非接触式电导检测和紫外（UV）检测的毛细管电泳来测定氨基酸（AA）和肽的工作，这是非毛细管电泳技术中最广泛使用的检测方法，无需柱前衍生化。描述了这些分析物的 UV 检测的可用选项，例如间接检测、与过渡金属离子络合和柱内衍生化。描述了直接检测具有紫外吸收的 AA 和肽以及手性分离方面的进展。单独的一节专门介绍了与毛细管电泳-UV 结合使用在线样品预浓缩方法。
