Department of Molecular Medicine, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran.
Department of Cellular and Molecular Biology, Faculty of Life Sciences and Biotechnologies, Shahid Beheshti University, Tehran, Iran.
Arch Microbiol. 2024 Aug 27;206(9):386. doi: 10.1007/s00203-024-04095-0.
The gram-negative bacterium Escherichia coli Nissle 1917 (EcN) has long been recognized for its therapeutic potential in treating various intestinal diseases. Bacterial ghosts (BGs) are empty shells of non-living bacterial cells that demonstrate enormous potential for medicinal applications. Genetic and chemical techniques can create these BGs. In the current study, we produced Escherichia coli Nissle 1917 ghosts (EcNGs) for the first time using benzoic acid (BA) and sodium hydroxide (SH). BA is a feeble acidic chemical that enhances gram-negative bacteria's external membrane permeability, reduces energy production, and decreases internal pH. SH has shown success in producing BGs from some gram-negative and gram-positive organisms. This research aims to produce EcNGs using the minimum inhibitory concentration (MIC) of SH and BA, specifically 3.125 mg/mL. We assessed the bacterial quality of the BGs produced using quantitative PCR (qPCR) and Bradford protein assays. Field emission scanning electron microscopy (FE-SEM) showed the three-dimensional structure of EcNGs. The study confirmed the presence of tunnel-like pores on the outer surface, indicating the preservation of cell membrane integrity. Importantly, this investigation introduces BA as a novel chemical inducer of EcNGs, suggesting its potential alongside SH for efficient EcNG formation.
革兰氏阴性菌大肠杆菌 Nissle 1917（EcN）长期以来因其在治疗各种肠道疾病方面的治疗潜力而受到关注。细菌体（BGs）是无生命细菌细胞的空壳，具有巨大的药用应用潜力。遗传和化学技术可以制造这些 BGs。在本研究中，我们首次使用苯甲酸（BA）和氢氧化钠（SH）生产大肠杆菌 Nissle 1917 体（EcNGs）。BA 是一种弱酸性化学物质，可增强革兰氏阴性菌的外膜通透性，降低能量产生，并降低内部 pH 值。SH 已成功用于从一些革兰氏阴性和革兰氏阳性生物中生产 BGs。本研究旨在使用 SH 和 BA 的最小抑菌浓度（MIC），即 3.125mg/mL，生产 EcNGs。我们使用定量 PCR（qPCR）和 Bradford 蛋白测定法评估 BGs 的细菌质量。场发射扫描电子显微镜（FE-SEM）显示了 EcNGs 的三维结构。该研究证实了外表面存在隧道样孔，表明细胞膜完整性得以保留。重要的是，这项研究将 BA 作为 EcNGs 的新型化学诱导剂引入，表明其与 SH 一起具有高效形成 EcNGs 的潜力。
