School of Public Health and Management, Weifang Medical University, Weifang, 261053, Shandong, China.
State Key Laboratory of Microbial Technology, National Glycoengineering Research Center, Shandong University, Qingdao, 266237, Shandong, China.
Microb Biotechnol. 2020 May;13(3):629-636. doi: 10.1111/1751-7915.13523. Epub 2019 Dec 21.
Bacterial vectors, as microscopic living 'robotic factories', can be reprogrammed into microscopic living 'robotic factories', using a top-down bioengineering approach to produce and deliver anticancer agents. Most of the current research has focused on bacterial species such as Salmonella typhimurium or Clostridium novyi. However, Escherichia coli Nissle 1917 (EcN) is another promising candidate with probiotic properties. EcN offers increased applicability for cancer treatment with the development of new molecular biology and complete genome sequencing techniques. In this review, we discuss the genetics and physical properties of EcN. We also summarize and analyse recent studies regarding tumour therapy mediated by EcN. Many challenges remain in the development of more promising strategies for combatting cancer with EcN.
细菌载体作为微观的活体“机器人工厂”，可以使用自上而下的生物工程方法进行重新编程，以产生和输送抗癌剂。目前的大多数研究都集中在细菌物种，如鼠伤寒沙门氏菌或诺维梭菌。然而，大肠杆菌 Nissle 1917（EcN）是另一种具有益生菌特性的有前途的候选者。随着新的分子生物学和完整基因组测序技术的发展，EcN 为癌症治疗提供了更大的适用性。在这篇综述中，我们讨论了 EcN 的遗传学和物理特性。我们还总结和分析了最近关于 EcN 介导的肿瘤治疗的研究。在利用 EcN 开发更有前途的抗癌策略方面，仍然存在许多挑战。
