Boyd A W, Fisher D C, Fox D A, Schlossman S F, Nadler L M
J Immunol. 1985 Apr;134(4):2387-92.
After activation, B cells express the IL 2 receptor as determined by their reactivity with monoclonal anti-IL 2 receptor antibodies. In this report we show that anti-IL 2 receptor antibodies precipitated comparable 60,000 to 65,000 dalton proteins from highly purified B and T cells. Limited peptide mapping suggested that the receptors on B and T cells were identical. Moreover, activated B cells could be induced to proliferate by IL 2, but not to secrete Ig. Anti-IL 2R antibody blocked the effect of IL 2 but not the proliferative response induced by B cell growth factor (BCGF), suggesting independent growth factor receptors. Investigation of the kinetics of the B cell response to growth factor indicated that BCGF acts within 24 hr, whereas IL 2 was virtually devoid of activity for 48 hr. Nevertheless, after 72 to 96 hr, the effect of IL 2 was equal to or greater than that obtained with BCGF. These studies suggest that the initial stages of B cell proliferation involves a sequential interaction of BCGF and IL 2 with their respective receptors.
激活后，通过B细胞与单克隆抗IL - 2受体抗体的反应性测定，发现B细胞表达IL - 2受体。在本报告中，我们表明抗IL - 2受体抗体从高度纯化的B细胞和T细胞中沉淀出了分子量相当的60,000至65,000道尔顿的蛋白质。有限的肽图谱分析表明B细胞和T细胞上的受体是相同的。此外，激活的B细胞可被IL - 2诱导增殖，但不分泌Ig。抗IL - 2R抗体可阻断IL - 2的作用，但不阻断B细胞生长因子（BCGF）诱导的增殖反应，提示存在独立的生长因子受体。对B细胞对生长因子反应动力学的研究表明，BCGF在24小时内起作用，而IL - 2在48小时内几乎没有活性。然而，在72至96小时后，IL - 2的作用等于或大于BCGF的作用。这些研究表明B细胞增殖的初始阶段涉及BCGF和IL - 2与其各自受体的顺序相互作用。
