Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy, Cairo University, El-Kasr El-Aini Street, Cairo, 11562, Egypt.
Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy, Al-Azhar University, Nasr City, Cairo, 11751, Egypt.
Sci Rep. 2024 Aug 27;14(1):19873. doi: 10.1038/s41598-024-69518-w.
There are no documented electroanalytical methods for quantifying the anti-inflammatory drug bumadizone (BUM) in pharmaceutical or biological matrices. So, a new voltammetric method was developed to determine BUM at nano concentrations in pharmaceutical forms, in the presence of its alkaline degradant, and in biological fluids. Five electrodes were tested, including three nano-reduced graphene oxide (nRGO) electrodes (5%, 15%, and 20%), a carbon paste electrode (CPE), and a 10% nRGO-modified CPE. The 10% nRGO-modified electrode showed the best performance, offering high selectivity and low detection limits, with good linearity in the concentration range of 0.9 × 10 to 15 × 10 ng mL. Differential pulse voltammetry successfully applied this electrode for BUM determination in various samples, achieving excellent recovery without preliminary separation. The method was validated according to ICH guidelines and compared favorably to the reference method. Its environmental impact was assessed using AGREE and Eco-scale metrics in addition to the RGB algorithm, showing superior greenness and whiteness profiles due to safer solvents and lower energy consumption, along with high practical effectiveness using the BAGI metric.
目前尚无文献报道可用于定量测定药物 bumadizone（BUM）的电化学分析方法。因此，开发了一种新的伏安法，用于在碱性降解产物存在下，在药物制剂中、在生物流体中测定毫微克浓度的 BUM。测试了五种电极，包括三种纳米还原氧化石墨烯（nRGO）电极（5%、15%和 20%）、碳糊电极（CPE）和 10% nRGO 修饰的 CPE。10% nRGO 修饰的电极表现出最佳性能，具有高选择性和低检测限，在浓度范围为 0.9×10 到 15×10ng mL 时具有良好的线性关系。差分脉冲伏安法成功地将该电极应用于各种样品中的 BUM 测定，无需初步分离即可获得优异的回收率。该方法根据 ICH 指南进行了验证，并与参考方法进行了比较。还使用 AGREE 和生态规模指标以及 RGB 算法评估了该方法的环境影响，由于使用更安全的溶剂和更低的能耗以及 BAGI 指标的高实际效果，该方法具有更好的绿色和白色轮廓。
