Britton Chance Center for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics, MoE Key Laboratory for Biomedical Photonics, Huazhong University of Science and Technology, Wuhan, 430074, China.
Key Laboratory of Biomedical Engineering of Hainan Province, School of Biomedical Engineering, Hainan University, Haikou, 570228, China.
Brain Struct Funct. 2024 Nov;229(8):2045-2057. doi: 10.1007/s00429-024-02851-y. Epub 2024 Aug 27.
The brain atlas is essential for exploring the anatomical structure and function of the brain. Non-human primates, such as cynomolgus macaque, have received increasing attention due to their genetic similarity to humans. However, current macaque brain atlases only offer coarse sections with intervals along the coronal direction, failing to meet the needs of single-cell resolution studies in functional and multi-omics research of the macaque brain. To address this issue, we utilized fluorescence micro-optical sectioning tomography to obtain sub-micron resolution cytoarchitectonic images of the macaque brain at the sagittal plane. Based on the obtained 8000 image sequences, a reference brain atlas comprising 45 sagittal sections was created, delineating 270 brain regions other than the cortex. Additionally, a website was established to share the reference atlas corresponding image data. This study is expected to provide an essential dataset and tool for scientists studying the macaque brain.
脑图谱对于探索大脑的解剖结构和功能至关重要。由于与人类具有较高的基因相似性，非人类灵长类动物（如食蟹猴）越来越受到关注。然而，目前的猴脑图谱仅提供沿冠状方向的粗略切片，无法满足猴脑功能和多组学研究中单细胞分辨率研究的需求。为了解决这个问题，我们利用荧光微光学切片层析成像技术，在矢状面获得了亚微米分辨率的猴脑细胞构筑图像。基于获得的 8000 个图像序列，创建了一个包含 45 个矢状切片的参考脑图谱，除了皮层外，还描绘了 270 个脑区。此外，还建立了一个网站来共享参考图谱的对应图像数据。这项研究有望为研究猴脑的科学家提供一个重要的数据集和工具。
