Jung T T, Berlinger N T, Juhn S K
Laryngoscope. 1985 Mar;95(3):307-12. doi: 10.1288/00005537-198503000-00014.
It has already been demonstrated in human and animal systems that PGE2 is a suppressor signal for many immune functions. These include T-lymphocyte blastogenesis, natural killer cell activity, and cytolytic T-lymphocyte activity. These functions are important for destruction of tumor cells. Conceivably, suppression of these functions by excessive PGE2 restricts tumor cell kill, and reversal of suppression by an inhibitor of prostaglandin synthesis such as indomethacin could increase tumor cell kill. The purpose of this study was to determine the kind of prostaglandins (PGs) produced by tissues with squamous cell carcinoma of head and neck and to measure the concentrations of PGE2, 6-keto-PGF1 alpha, and thromboxane (Tx) B2 in the tumor tissue and in the corresponding control tissue. Tumor and normal control tissues at the margin of the resection were obtained from surgical specimens. The production of PGs was determined by incubation of tissue homogenates with 14C-arachidonic acid, by thin layer chromatography, autoradiography, and scintillation counting. Concentrations of PGs were measured by radioimmunoassay. Tumor tissues produced PGD2, E2, TxB2, F2 alpha, and 6-keto-F1 alpha, and 15-, 12-, and 5-monohydroxyeicosatetraenoic acid (HETE). Concentrations of PGE2 were four times higher in the tumor tissues compared to those in control tissues. There was no difference between the levels of TxB2 and 6-keto-PGF1 alpha in the tumor tissues and those in control tissues. The results of this study will serve as basic information necessary for the potential use of inhibitors of PG-synthesis in the treatment of head and neck carcinoma.
在人类和动物系统中已经证实，前列腺素E2（PGE2）是多种免疫功能的抑制信号。这些免疫功能包括T淋巴细胞增殖、自然杀伤细胞活性和细胞毒性T淋巴细胞活性。这些功能对于肿瘤细胞的破坏很重要。可以想象，过量的PGE2对这些功能的抑制会限制肿瘤细胞的杀伤，而通过前列腺素合成抑制剂（如消炎痛）来逆转这种抑制可能会增加肿瘤细胞的杀伤。本研究的目的是确定头颈部鳞状细胞癌组织产生的前列腺素（PGs）种类，并测量肿瘤组织和相应对照组织中PGE2、6-酮-前列腺素F1α和血栓素（Tx）B2的浓度。手术标本取自切除边缘的肿瘤组织和正常对照组织。通过将组织匀浆与14C-花生四烯酸一起孵育、薄层色谱法、放射自显影和闪烁计数来确定PGs的产生。通过放射免疫测定法测量PGs的浓度。肿瘤组织产生前列腺素D2、E2、血栓素B2、F2α和6-酮-F1α，以及15-、12-和5-单羟基二十碳四烯酸（HETE）。与对照组织相比，肿瘤组织中PGE2的浓度高出四倍。肿瘤组织中血栓素B2和6-酮-前列腺素F1α的水平与对照组织中的水平没有差异。本研究结果将作为在头颈部癌治疗中潜在使用PG合成抑制剂所需的基础信息。
