Department of Chemistry, College of Science, Princess Nourah bint Abdulrahman University, Riyadh, Saudi Arabia.
Department of Chemistry, College of Science, University of Jeddah, Jeddah, Saudi Arabia.
Luminescence. 2024 Sep;39(9):e4882. doi: 10.1002/bio.4882.
Aspartame is an artificial sweetener used in drinks and many foods. International Agency for Research on Cancer classified aspartame as possibly carcinogenic to humans (IARC Group 2B). In this study, a sensitive and selective spectrofluorimetric method was developed to detect aspartame. The method is based on switching on the fluorescence activity of aspartame upon its condensation with O-phthalaldehyde (Roth's reaction) in the presence of 2-mercaptoethanol. The reaction product was detected fluorometrically at λ of 438 nm after λ of 340 nm. All reaction conditions required to yield the optimal fluorescence intensity were observed and investigated. Furthermore, the approach was validated according to ICH guidelines. Upon plotting the concentrations of aspartame against their associated fluorescence intensity values, the relationship between the two variables was linear within the range of 0.5-3.0 μg/mL. Furthermore, the method was employed to analyze the quantity of aspartame in commercial packages and soft drinks with an acceptable level of recovery. In addition, the Green Solvents Selecting Tool, Complementary Green Analytical Procedure Index, and the Analytical Greenness Metric tool were used to evaluate the sustainability and the greenness of the developed methodology.
阿斯巴甜是一种用于饮料和许多食品的人工甜味剂。国际癌症研究机构将阿斯巴甜归类为可能对人类致癌物质（国际癌症研究机构 2B 组）。在这项研究中，开发了一种灵敏和选择性的荧光分光光度法来检测阿斯巴甜。该方法基于在存在 2-巯基乙醇的情况下，使阿斯巴甜与邻苯二醛（Roth 反应）缩合，从而开启其荧光活性。在 λ 为 340nm 后，在 λ 为 438nm 处检测到反应产物的荧光。观察和研究了产生最佳荧光强度所需的所有反应条件。此外，该方法根据 ICH 指南进行了验证。通过将阿斯巴甜的浓度与它们相关的荧光强度值作图，可以在 0.5-3.0μg/mL 的范围内观察到两个变量之间的线性关系。此外，该方法用于分析商业包装和软饮料中阿斯巴甜的含量，回收率可接受。此外，使用绿色溶剂选择工具、补充绿色分析程序指数和分析绿色度工具来评估所开发方法的可持续性和绿色性。
