Yang Zhongzhou, Jin Wei, Luo Qi, Li Xiaoli, Wei Yunmin, Lin Yunlong
Key Laboratory of Plant Genetics and Molecular Breeding, Zhoukou Normal University, Zhoukou 466001, China.
College of Life Sciences and Oceanography, Shenzhen University, Shenzhen 518060, China.
Biology (Basel). 2024 Jul 23;13(8):556. doi: 10.3390/biology13080556.
Acting as the most abundant and widely distributed volatile secondary metabolites in plants, terpenoids play crucial roles in diverse physiological regulations and metabolic processes. Terpene synthases play a decisive role in determining the composition and diversity of terpenoids. Though the regulation of terpene synthases has been extensively investigated across various plant species, limited studies have focused on the upstream transcriptional regulation of terpene synthases. In this study, we have identified linalool as the predominant volatile compound that is released gradually from flowers throughout flower blooming. In the context of the transcriptome, a typical MYB transcription factor, FhMYB108, was screened based on homologous gene comparison. is capable of regulating the expression of , and both their expression levels showed gradual increase during flower opening. Moreover, exerts a stimulatory effect on the transcription of , while no significant increase in expression is observed upon the stabilization of in . The highly expressed in could interact with to suppress the activation of by . The present study not only elucidates the regulatory mechanism underlying linalool synthesis but also discovers the synergistic effect of MYB and bHLH transcription factors in governing the biosynthesis of volatile terpenoids.
萜类化合物作为植物中含量最丰富、分布最广泛的挥发性次生代谢产物，在多种生理调节和代谢过程中发挥着关键作用。萜烯合酶在决定萜类化合物的组成和多样性方面起着决定性作用。尽管在各种植物物种中对萜烯合酶的调控已进行了广泛研究，但对萜烯合酶上游转录调控的研究较少。在本研究中，我们已确定芳樟醇是在整个花朵开放过程中从花朵中逐渐释放的主要挥发性化合物。在转录组的背景下，基于同源基因比较筛选出一个典型的MYB转录因子FhMYB108。它能够调节[具体基因1]的表达，并且它们的表达水平在花朵开放期间均呈逐渐增加。此外，[具体基因2]对[具体基因1]的转录具有刺激作用，而当[具体基因2]在[具体条件]下稳定时，未观察到[具体基因3]表达的显著增加。在[具体植物]中高表达的[具体基因4]可与[具体基因5]相互作用，以抑制[具体基因6]对[具体基因7]的激活。本研究不仅阐明了芳樟醇合成的调控机制，还发现了MYB和bHLH转录因子在控制挥发性萜类化合物生物合成中的协同作用。
