Zeng Xiaoyan, Tong Li
Qinghai University, Xining 810000, China.
Qinghai Provincial Key Laboratory of Traditional Chinese Medicine Research for Glucolipid Metabolic Diseases, Xining 810000, China.
Biology (Basel). 2024 Jul 24;13(8):557. doi: 10.3390/biology13080557.
The increasing prevalence of diabetic reproductive complications has prompted the development of innovative animal models. The use of the silkworm as a model for diabetic reproductive damage shows potential as a valuable research tool. This study employed silkworms as a novel model to investigate diabetic reproductive damage. The silkworms were fed a high-glucose diet containing 10% glucose to induce a diabetic model. Subsequently, the study concentrated on assessing the influence of diabetes on the reproductive system of male silkworms. The results indicate that diabetes resulted in reduced luteinizing hormone (LH) and testosterone (T) levels, as well as elevated triglyceride (TG) levels in male silkworms. Moreover, diabetes mellitus was associated with pathological testicular damage in male silkworms, accompanied by decreased glutathione peroxidase (GSH-Px) and superoxide dismutase (SOD) levels, along with increased malondialdehyde (MDA) levels in the testis. Additionally, diabetes mellitus reduced the expression of siwi1 and siwi2 genes in the testis of male silkworms. Overall, these results support using silkworms as a valuable model for studying diabetic reproductive damage.
糖尿病生殖并发症患病率的不断上升促使了创新动物模型的开发。将家蚕用作糖尿病生殖损伤模型显示出作为一种有价值研究工具的潜力。本研究采用家蚕作为新型模型来研究糖尿病生殖损伤。给家蚕喂食含10%葡萄糖的高糖饮食以诱导糖尿病模型。随后，该研究集中评估糖尿病对雄蚕生殖系统的影响。结果表明，糖尿病导致雄蚕促黄体生成素（LH）和睾酮（T）水平降低，以及甘油三酯（TG）水平升高。此外，糖尿病与雄蚕睾丸病理损伤有关，伴有睾丸中谷胱甘肽过氧化物酶（GSH-Px）和超氧化物歧化酶（SOD）水平降低，以及丙二醛（MDA）水平升高。此外，糖尿病降低了雄蚕睾丸中siwi1和siwi2基因的表达。总体而言，这些结果支持将家蚕用作研究糖尿病生殖损伤的有价值模型。
