Kim Hyunjung, Kim Boyong
Department of Health and Safety Convergence Science, Korea University, Anam-ro 145, Seongbuk-gu, Seoul 02841, Republic of Korea.
EVERBIO, 131, Jukhyeon-gil, Gwanghyewon-myeon, Jincheon-gun 27809, Republic of Korea.
J Funct Biomater. 2024 Jul 31;15(8):215. doi: 10.3390/jfb15080215.
Fine dust causes various disorders, including cardiovascular, neurological, renal, reproductive, motor, systemic, respiratory, and cancerous diseases. Therefore, it is essential to study functional materials to prevent these issues. This study investigated the beneficial effects of erucic acid against fine dust using methods such as miRNA profiling, quantitative PCR, flow cytometry, ELISA, and Alizarin O staining. Erucic acid effectively suppresses inflammation and upregulates osteogenic activators in fibroblasts exposed to fine dust. Additionally, erucic acid-induced exosomes (EIEs) strongly counteract the negative effects of fine dust on osteocytic differentiation and inflammation. Despite fine dust exposure, EIEs promoted osteocytic differentiation in adipose-derived stem cells (ASCs) and enhanced osteogenesis and phagocytosis in macrophages. The significant upregulation of RunX2 and BMP7 by EIEs indicates its strong role in osteocytic differentiation and protection against the effects of fine dust. EIEs also boosts immune activity and acts as an osteogenic trigger for macrophages. MicroRNA profiling revealed that EIEs dramatically upregulated miRNAs, including hsa-miRNA-1301-3p, hsa-miRNA-1908-5p, hsa-miRNA-423-5p, and hsa-miRNA-122-5p, which are associated with osteogenic differentiation and immunity. Therefore, EIEs show potential as biomaterials to prevent environment-borne diseases.
细颗粒物会引发多种疾病，包括心血管疾病、神经疾病、肾脏疾病、生殖疾病、运动系统疾病、全身性疾病、呼吸系统疾病和癌症。因此，研究功能性材料以预防这些问题至关重要。本研究采用微小RNA分析、定量聚合酶链反应、流式细胞术、酶联免疫吸附测定和茜素O染色等方法，研究了芥酸对细颗粒物的有益作用。芥酸可有效抑制炎症，并上调暴露于细颗粒物的成纤维细胞中的成骨激活因子。此外，芥酸诱导的外泌体（EIEs）能强烈抵消细颗粒物对骨细胞分化和炎症的负面影响。尽管暴露于细颗粒物中，但EIEs促进了脂肪来源干细胞（ASCs）的骨细胞分化，并增强了巨噬细胞的成骨作用和吞噬作用。EIEs使RunX2和BMP7显著上调，表明其在骨细胞分化和抵御细颗粒物影响方面发挥着重要作用。EIEs还能增强免疫活性，并作为巨噬细胞的成骨触发因素。微小RNA分析显示，EIEs显著上调了包括hsa-miRNA-1301-3p、hsa-miRNA-1908-5p、hsa-miRNA-423-5p和hsa-miRNA-122-5p在内的微小RNA，这些微小RNA与成骨分化和免疫相关。因此，EIEs作为预防环境传播疾病的生物材料具有潜力。
