BIOMIT Lab (Biomaterials in Tissue Engineering Lab) Institute of Physics La Plata (IFLP), University of La Plata & CONICET, Diag. 113 e/63 y 64, CP 1900, La Plata, Buenos Aires, Argentina.
Department of Biomaterials, Celll Institute, Ciudad de Buenos Aires, Argentina.
J Mater Chem B. 2023 Aug 24;11(33):7998-8006. doi: 10.1039/d3tb00717k.
There are many challenges in the development of 3D-tissue models for studying bone physiology and disease. Silk fibroin (SF), a natural fibrous protein used in biomedical applications has been studied for bone tissue engineering (TE) due to its mechanical properties, biocompatibility and biodegradability. However, low osteogenic capacity as well as the necessity to reinforce the protein mechanically for some orthopedic applications prompts the need for further designs for SF-based materials for TE bone. Concentric mineralized porous SF-based scaffolds were developed to improve mechanics and mineralization towards osteoregeneration. Hybrid SF silica microparticles (MP) or calcium carbonate nano-structured microparticles (NMP) were seeded with hMSCs co-cultured under osteogenic and osteoclastic conditions with THP-1 human monocytes up to 10 weeks to simulate and recapitulate bone regeneration. Scaffolds with appropriate pore size for cell infiltration, resulted in improved compressive strength, increased cell attachment and higher levels of expression of osteogenic markers and mineralization after adding the NMPs, compared to controls systems without these particles. These hybrid SF-based 3D-structures can provide improved scaffold designs for bone TE.
用于研究骨生理学和疾病的 3D 组织模型的发展存在许多挑战。丝素蛋白（SF）是一种用于生物医学应用的天然纤维状蛋白质，由于其机械性能、生物相容性和可生物降解性，已被用于骨组织工程（TE）研究。然而，低成骨能力以及一些矫形应用中需要增强蛋白质的机械强度，促使需要对基于 SF 的材料进行进一步设计，以用于 TE 骨。同心矿化多孔 SF 基支架的开发是为了改善力学性能和矿化以促进骨再生。将 hMSCs 与 THP-1 人单核细胞共培养，在成骨和破骨条件下，接种到 SF 混合二氧化硅微球（MP）或碳酸钙纳米结构微球（NMP）中，共培养长达 10 周，以模拟和再现骨再生。与没有这些颗粒的对照体系相比，具有适当孔径以利于细胞渗透的支架可提高抗压强度、增加细胞附着，并提高成骨标志物和矿化的表达水平。这些基于 SF 的混合 3D 结构可为骨 TE 提供改进的支架设计。
