Xu Xiaoming, Yu Chong, Liu Zhenyang, Cui Xiaohang, Guo Xiaohe, Wang Haifeng
Key Laboratory of Structure-Based Drug Design & Discovery of Ministry of Education, School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, Shenyang 110016, China.
Guangxi Key Laboratory of Marine Natural Products and Combinatorial Biosynthesis Chemistry, Guangxi Academy of Sciences, Nanning 530007, China.
J Fungi (Basel). 2024 Aug 5;10(8):552. doi: 10.3390/jof10080552.
Shiitake mushrooms () are renowned as the "King of mountain treasures" in China due to their abundant nutritional and health-enhancing properties. Intensive chemical investigations of the fruiting bodies and mycelium of Shiitake mushrooms () afforded five new compounds (), named lentinmacrocycles A-C and lentincoumarins A-B, along with fifteen known compounds (). Their structures and absolute configurations were elucidated by extensive spectroscopic analysis, including one-and two-dimensional (1D and 2D) NMR spectroscopy, circular dichroism (CD), and high-resolution electrospray ionization mass spectrometry (HR-ESI-MS). The anti-inflammatory activity test showed that lentincoumarins A (), (3)-7-hydroxymellein (), (3)-6-hydroxymellein () and succinic acid () exhibited strong NO inhibitory effects (IC < 35 μM), and that (3)-5-hydroxymellein () and (3)-6-hydroxymellein () exhibited potent TNF-α inhibitory effects (IC < 80 μM) and were more potent than the positive control, Indomethacin (IC = 88.5 ± 2.1 μM). The antioxidant activity test showed that (3)-6-hydroxymellein () had better DPPH radical scavenging activity (IC = 25.2 ± 0.5 μM).
香菇在中国被誉为“山珍之王”，因其具有丰富的营养和增进健康的特性。对香菇子实体和菌丝体进行深入的化学研究，得到了五种新化合物，命名为香菇大环内酯A - C和香菇香豆素A - B，以及十五种已知化合物。通过广泛的光谱分析，包括一维和二维（1D和2D）核磁共振光谱、圆二色性（CD）和高分辨率电喷雾电离质谱（HR - ESI - MS），阐明了它们的结构和绝对构型。抗炎活性测试表明，香菇香豆素A、（3） - 7 - 羟基水合氧化麦角硫因、（3） - 6 - 羟基水合氧化麦角硫因和琥珀酸表现出强烈的NO抑制作用（IC < 35 μM），并且（3） - 5 - 羟基水合氧化麦角硫因和（3） - 6 - 羟基水合氧化麦角硫因表现出有效的TNF - α抑制作用（IC < 80 μM），且比阳性对照吲哚美辛（IC = 88.5 ± 2.1 μM）更有效。抗氧化活性测试表明，（3） - 6 - 羟基水合氧化麦角硫因具有更好的DPPH自由基清除活性（IC = 25.2 ± 0.5 μM）。
