Zheng Xu, Li Ao, Nie Ruining, Wu Chengxu, Ji Xinying, Tang Jiali, Zhang Junpei
State Key Laboratory of Tree Genetics and Breeding, Key Laboratory of Tree Breeding and Cultivation of the State Forestry and Grassland Administration, Forestry Research Institute of China Academy of Forestry, State Forestry and Grassland Administration, Beijing 100091, China.
J Fungi (Basel). 2024 Aug 6;10(8):554. doi: 10.3390/jof10080554.
To delve into the growth and physiological adaptations exhibited by the economically vital black wolfberry () upon inoculation with arbuscular mycorrhizal fungi (AMF) under varying levels of saline-alkaline stress A series of pot experiments were conducted in a gradient saline-alkaline environment (0, 200, 400 mM NaCl: NaHCO = 1:1). One-year-old cuttings of black wolfberry, inoculated with two AMF species-Funneliformis mosseae (Fm) and Rhizophagus intraradices (Ri)-served as the experimental material, enabling a comprehensive analysis of seedling biomass, chlorophyll content, antioxidant enzyme activities, and other crucial physiological parameters. This study demonstrated that both Fm and Ri could form a symbiotic relationship with the root of . Notably, Fm inoculation significantly bolstered the growth of the underground parts, while exhibiting a remarkable capacity to scavenge reactive oxygen species (ROS), thereby effectively mitigating membrane oxidative damage induced by stress. Additionally, Fm promoted the accumulation of abscisic acid (ABA) in both leaves and roots, facilitating the exclusion of excess sodium ions from cells. Ri Inoculation primarily contributed to an enhancement in the chlorophyll b (Chlb) content, vital for sustaining photosynthesis processes. Furthermore, Ri's ability to enhance phosphorus (P) absorption under stressful conditions ensured a steady influx of essential nutrients. These findings point to different strategies employed for Fm and Ri inoculation. To holistically assess the saline-alkaline tolerance of each treatment group, a membership function analysis was employed, ultimately ranking the salt tolerance as Fm > Ri > non-mycorrhizal (NM) control. This finding holds paramount importance for the screening of highly resilient strains and offers invaluable theoretical underpinnings and technical guidance for the remediation of saline-alkaline soils, fostering sustainable agricultural practices in challenging environments.
为深入研究经济价值重要的黑枸杞在不同盐碱胁迫水平下接种丛枝菌根真菌（AMF）后的生长及生理适应性，在梯度盐碱环境（0、200、400 mM NaCl:NaHCO₃ = 1:1）中进行了一系列盆栽试验。以接种两种AMF菌种——摩西管柄囊霉（Fm）和根内根孢囊霉（Ri）的一年生黑枸杞插条作为试验材料，全面分析幼苗生物量、叶绿素含量、抗氧化酶活性及其他关键生理参数。本研究表明，Fm和Ri均可与黑枸杞根系形成共生关系。值得注意的是，接种Fm显著促进了地下部分的生长，同时表现出显著的清除活性氧（ROS）的能力，从而有效减轻胁迫诱导的膜氧化损伤。此外，Fm促进了叶片和根系中脱落酸（ABA）的积累，有助于细胞排出过量的钠离子。接种Ri主要促进了叶绿素b（Chlb）含量的增加，这对维持光合作用过程至关重要。此外，Ri在胁迫条件下增强磷（P）吸收的能力确保了必需养分的稳定流入。这些发现表明Fm和Ri接种采用了不同的策略。为全面评估各处理组的盐碱耐受性，采用了隶属函数分析，最终将耐盐性排序为Fm＞Ri＞非菌根（NM）对照。这一发现对于筛选高抗性菌株至关重要，并为盐碱土壤修复提供了宝贵的理论基础和技术指导，促进了在具有挑战性环境中的可持续农业实践。
