Hammad Alaa M, Alzaghari Lujain F, Alfaraj Malek, Lux Vanessa, Sunoqrot Suhair
Department of Pharmacy, Faculty of Pharmacy, Al-Zaytoonah University of Jordan, Amman 11733, Jordan.
Department of Genetic Psychology, Ruhr University Bochum, 44801 Bochum, Germany.
Toxics. 2024 Aug 18;12(8):598. doi: 10.3390/toxics12080598.
Repeated exposure to tobacco smoke causes neuroinflammation and neuroplasticity, which correlates with smoking withdrawal-induced anxiety. The purpose of this study was to investigate the anticipated involvement of antioxidant-rich nanoparticles (NPs) prepared by oxidation-triggered polymerization of green tea catechins in impacting these effects in a rat model of tobacco smoke exposure. Exposure to tobacco smoke was carried out for 2 h a day, 5 days a week, for a total of 36 days. Weekly behavioral tests were conducted prior to recommencing the exposure. Following a 20-day exposure period, rats were administered either distilled water or green tea (GT) NPs (20 mg/kg, orally) for an additional 16 days. Our findings revealed that tobacco smoke exposure induced anxiety-like behavior indicative of withdrawal, and this effect was alleviated by GT NPs. Tobacco smoke exposure caused a marked increase in the relative mRNA and protein expression of nuclear factor-kappa B (NF-κB) and reduced the relative mRNA and protein expression of brain-derived neurotrophic factor (BDNF) in the hippocampus (HIP) and hypothalamus (HYP) brain subregions. The intervention of GT NPs effectively inhibited these effects. Our findings demonstrate the potent protective role of GT NPs in reducing withdrawal-induced anxiety-like behavior, neuroinflammation, and neuroplasticity triggered by tobacco smoke exposure.
反复接触烟草烟雾会导致神经炎症和神经可塑性，这与戒烟引起的焦虑有关。本研究的目的是调查通过绿茶儿茶素氧化引发聚合制备的富含抗氧化剂的纳米颗粒（NPs）在烟草烟雾暴露大鼠模型中对这些影响的预期作用。每天暴露于烟草烟雾2小时，每周5天，共36天。在重新开始暴露之前每周进行行为测试。在20天的暴露期后，大鼠再额外接受16天的蒸馏水或绿茶（GT）纳米颗粒（20mg/kg，口服）。我们的研究结果表明，烟草烟雾暴露会诱发类似戒断的焦虑行为，而GT纳米颗粒可减轻这种效应。烟草烟雾暴露导致海马体（HIP）和下丘脑（HYP）脑区中核因子κB（NF-κB）的相对mRNA和蛋白质表达显著增加，并降低脑源性神经营养因子（BDNF）的相对mRNA和蛋白质表达。GT纳米颗粒的干预有效抑制了这些效应。我们的研究结果证明了GT纳米颗粒在减少烟草烟雾暴露引发的戒断诱导的焦虑样行为、神经炎症和神经可塑性方面具有强大的保护作用。
