El-Sayed Ahmed, Faraj Salah H, Marghani Basma H, Safhi Fatmah A, Abdo Mohamed, Fericean Liana, Banatean-Dunea Ioan, Alexandru Cucui-Cozma, Alhimaidi Ahmad R, Ammari Aiman A, Eissa Attia, Ateya Ahmed
Department of Animal Health and Poultry, Animal and Poultry Production Division, Desert Research Center (DRC), Cairo 11753, Egypt.
Department of Biology, College of Science, University of Misan, Maysan 62001, Iraq.
Vet Sci. 2024 Jul 27;11(8):340. doi: 10.3390/vetsci11080340.
Determining the gene expression and serum profile of the indicators linked to clinical endometritis susceptibility in Egyptian buffalo cows was the aim of this investigation. The buffalo cows that were enrolled were divided into two groups: forty infected buffalo cows with clinical endometritis and forty seemingly healthy buffalo cows that served as the control group. For the purposes of gene expression and biochemical analysis, ten milliliters of blood was obtained via jugular venipuncture from each buffalo cow. , , , , , , , , , , and were manifestly expressed at much higher levels in the buffaloes with endometritis. On the other hand, the genes that encode , , , , and were down-regulated. There was a significant ( < 0.05) elevation of the serum levels of non-esterified fatty acids (NEFAs), beta hydroxy butyric acid (BHBA), triglycerides (TGs), globulin, creatinine, and cortisol, along with a reduction in the serum levels of glucose, cholesterol, total protein albumin, urea, estrogen (E2), progesterone (P4), follicle-stimulating hormone (FSH), luteinizing hormone (LH), thyroxine (T4), prostaglandin F2 α (PGF2α), calcium, iron, and selenium, in the endometritis group in comparison with the control. However, no significant change was observed in the values of phosphorus, magnesium, copper, or zinc in either group. Within the selective breeding of naturally resistant animals, the variation in the genes under study and the changes in the serum profiles of the indicators under investigation may serve as a reference guide for reducing endometritis in Egyptian buffalo cows.
本研究的目的是确定与埃及水牛临床子宫内膜炎易感性相关指标的基因表达和血清谱。纳入的水牛分为两组：40头患有临床子宫内膜炎的感染水牛和40头看似健康的水牛作为对照组。为了进行基因表达和生化分析，通过颈静脉穿刺从每头水牛采集10毫升血液。在患有子宫内膜炎的水牛中，[具体基因名称1]、[具体基因名称2]、[具体基因名称3]、[具体基因名称4]、[具体基因名称5]、[具体基因名称6]、[具体基因名称7]、[具体基因名称8]、[具体基因名称9]、[具体基因名称10]明显表达水平更高。另一方面，编码[具体基因名称11]、[具体基因名称12]、[具体基因名称13]、[具体基因名称14]和[具体基因名称15]的基因下调。与对照组相比，子宫内膜炎组血清中非酯化脂肪酸（NEFAs）、β-羟基丁酸（BHBA）、甘油三酯（TGs）、球蛋白、肌酐和皮质醇水平显著升高（P<0.05），同时血清中葡萄糖、胆固醇、总蛋白白蛋白、尿素、雌激素（E2）、孕酮（P4）、促卵泡激素（FSH）、促黄体生成素（LH）、甲状腺素（T4）、前列腺素F2α（PGF2α）、钙、铁和硒水平降低。然而，两组中磷、镁、铜或锌的值均未观察到显著变化。在自然抗性动物的选择性育种中，所研究基因的变异和所调查指标血清谱的变化可为降低埃及水牛子宫内膜炎提供参考指导。
